S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) The Seoul Journal of Medicine The Seoul Journal of Medicine Vol. 32 No.3 (1991)
Study of the Normal Development of Rat Palatal Rugae and the Role of Collagen Fibers in Rugae Development
흰쥐 태자에서 구개주름의 발생과 이에 미치는 아교섬유의 역할에 관한 연구
- Hwang, Young-il; Lee, Wang Jae; Lee, Eun Young; Lee, Kwang Ho; Chang, Ka Young
- Issue Date
- Seoul J Med, Vol.32 No.3, pp. 107-119
- Rat; Fetus; Rugaepalatinae; Basement membrane; Mesenchymal cells; Collagen fibers; Organ culture; beta-aminopropionitrile (BAPN)
- Sprague-Dawley rat fetuses ranging from 15- to 19-day of gestational age,
were studied with their rugae morphogenesis at the light microscopic level. The distribution
of collagen fibers in the palatal mesenchyme was analyzed. In addition, an attempt
to evaluate the role of collagen fibers in rugae development was made. The specimens
were H-E stained and collagen fiber stained according to the Gorden and Sweet method.
To evaluate the role of collagen fibers in rugae formation, p-aminopropionitrile (BAPN),
a well-known lathyrogen, was orally administered to the pregnant rats. Concurrently,
an organ culture of the fetal palate was taken with and without BAPN in the culture
media. Rugae in the palate had maturated anteroposteriorly in general. Individual ruga
development was accompanied by local epithelial cell proliferation, changes in the position
of the basement membrane, and crowding and rearrangement of the mesenchymal
cells. Collagen fibers were also accumulated beneath the basement membrane of the
rugae with changes of their arrangement pattern from reticular to concentric form.
The palatal processes of a 15-day-old fetuses cultured with BAPN showed some mesenchymal
invasion by proliferating epithelial cells of rugae. In contrast, the cultured palate
of the 17-day-old fetus with BAPN showed an exaggerated protruding of rugae above
the epithelial surface.
Following the above procedures, some descriptions of rugae development in rat fetuses
were obtained. With these results, it was suggested that mesenchymal cells and collagen
fibers may play important roles in rugae formation, and the contrasting results of the
organ culture experiment with 15- and 17-day-old fetuses might mean that collagen
fibers have the possibility of not inducing but counterbalancing normal development
of rugae in rat fetuses.