S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) The Seoul Journal of Medicine The Seoul Journal of Medicine Vol. 32 No.3 (1991)
Amplification of VNTR Locus D1S80 in DNA Recovered from Vaginal Sperm
질내정자에서 추출한 DNA에서 D1S80의 PCR 증폭
- Lee, Jung Bin
- Issue Date
- Seoul J Med, Vol.32 No.3, pp. 149-154
- Sperm DNA was isolated from vaginal fluid and/or endocervical mucoid
plugs at various postcoital intervals. Samples were obtained from 25 volunteer couples.
DNA was also prepared from the blood and semen of the couples. The DNA was amplified
by polymerase chain reaction (peR) with D1S80 primers. The Fragment length
polymorphism of the amplified products (AMP-FLP) was analysed by acrylamide gel
electrophoresis followed by ethidium bromide staining. The AMP-FLP of the vaginal
fluid was identical to that of the blood of the male partner within 10 hours after coitus.
Thereafter, the male DNA recovery rate reduced as the collection interval extended.
After 76 hours no male DNA profile was detected in the vaginal sample. Therefore,
AMP-FLP analysis of the D1S80 can be used to exclude to determine the probable
identity of an assailant in rape cases.