Publications
Detailed Information
A potential role for skeletal muscle caveolin-1 as an insulin sensitivity modulator in ageing-dependent non-obese type 2 diabetes: studies in a new mouse model
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Oh, Y S | - |
dc.contributor.author | Khil, L-Y | - |
dc.contributor.author | Cho, K A | - |
dc.contributor.author | Ryu, S J | - |
dc.contributor.author | Ha, M K | - |
dc.contributor.author | Cheon, G J | - |
dc.contributor.author | Lee, T S | - |
dc.contributor.author | Yoon, J-W | - |
dc.contributor.author | Jun, H-S | - |
dc.contributor.author | Park, S C | - |
dc.date.accessioned | 2010-06-07T03:29:11Z | - |
dc.date.available | 2010-06-07T03:29:11Z | - |
dc.date.issued | 2008-04-15 | - |
dc.identifier.citation | Diabetologia 51(6):1025-1034 | en |
dc.identifier.issn | 0012-186X (Print) | - |
dc.identifier.uri | http://www.springerlink.com/content/q485m1k10q73nq76/fulltext.pdf | - |
dc.identifier.uri | https://hdl.handle.net/10371/67499 | - |
dc.description.abstract | AIMS/HYPOTHESIS: Type 2 diabetes mellitus is a common age-dependent disease. We discovered that male offspring of non-diabetic C57BL/6 and DBA/2 mice, called JYD mice, develop type 2 diabetes when they grow old. JYD mice show characteristics of insulin resistance, hyperglycaemia and hyperinsulinaemia in old age without obesity. We postulated that the mechanism of age-dependent type 2 diabetes in this model relates to caveolin-1 status in skeletal muscle, which appears to regulate insulin sensitivity in the mice. METHODS: We compared insulin sensitivity in aged C57BL/6 and JYD mice using glucose and insulin tolerance tests and (18)F-fluorodeoxyglucose positron emission tomography. We also determined insulin signalling molecules and caveolin proteins using western blotting, and altered caveolin-1 levels in skeletal muscle of C57BL/6 and JYD mice using viral vector systems, to examine the effect of this on insulin sensitivity. RESULTS: In 30-week-old C57BL/6 and JYD mice, the basal levels of IRS-1, Akt and peroxisome proliferator-activated receptor-gamma decreased, as did insulin-stimulated phosphorylation of Akt and insulin receptor beta. However, caveolin-1 was only increased about twofold in 30-week-old JYD mice as compared with 3-week-old mice, whereas an eightfold increase was seen in C57BL/6 mice. Downregulation of caveolin-1 production in C57BL/6 mice caused severe impairment of glucose and insulin tolerance. Upregulation of caveolin-1 in aged diabetic JYD mice significantly improved insulin sensitivity with a concomitant increase of glucose uptake in the skeletal muscle. CONCLUSIONS/INTERPRETATION: The level of skeletal muscle caveolin-1 is correlated with the progression of age-dependent type 2 diabetes in JYD mice. | en |
dc.language.iso | en | en |
dc.publisher | Springer Verlag | en |
dc.subject | Aging/*physiology | en |
dc.subject | Animals | en |
dc.subject | Biological Transport | en |
dc.subject | Blood Glucose/drug effects/metabolism | en |
dc.subject | Caveolin 1/*physiology | en |
dc.subject | Crosses, Genetic | en |
dc.subject | Diabetes Mellitus, Type 2/*physiopathology | en |
dc.subject | Disease Models, Animal | en |
dc.subject | Female | en |
dc.subject | Fluorodeoxyglucose F18/metabolism | en |
dc.subject | Glucose Tolerance Test | en |
dc.subject | Insulin/pharmacology | en |
dc.subject | Male | en |
dc.subject | Mice | en |
dc.subject | Mice, Inbred C57BL | en |
dc.subject | Mice, Inbred DBA | en |
dc.subject | Muscle, Skeletal/*physiopathology | en |
dc.subject | Positron-Emission Tomography | en |
dc.title | A potential role for skeletal muscle caveolin-1 as an insulin sensitivity modulator in ageing-dependent non-obese type 2 diabetes: studies in a new mouse model | en |
dc.type | Article | en |
dc.identifier.doi | 10.1007/s00125-008-0993-0 | - |
- Appears in Collections:
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.