S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) The Seoul Journal of Medicine The Seoul Journal of Medicine Vol. 32 No.3 (1991)
A New Technique for the Cytogenetic Study of Human Oocytes
인간 난자의 세포유전학적 연구를 위한 염색체 분석법의 개발
- Pang, Myung-Geol; Oh, Sun Kyung; Hwang, Do Yeong; Moon, Shin Yong; Lee, Jin Yong; Chang, Yoon Seok; Choi, Kyoo Wan
- Issue Date
- Seoul J Med, Vol.32 No.3, pp. 155-159
- Oocyte chromosome; Inseminated human oocytes; Aneuploidy; Premature chromosome condensation
- A technique for making good quality chromosome preparations from human
oocytes is described. Analyzable good Quality chromosomes were present in human
oocytes that were hypotonically treated and fixed.
Ninety-eight oocytes were processed. Chromosome analysis could be performed on
80 (82%) oocytes. Seven oocytes showed an absence of chromosomes, mainly due to
degeneration. Chromosome analysis could not be performed on 11 oocytes because
the chromosome number could not be determined. Of 80 analyzable preparations, a
63% (50) incidence of aneuploidy with/without premature chromosome condensation
was observed in the human oocytes that failed to fertilize in vitro with 3:4 ratios of
hypo and hyperhaploidy.
Our method presented in this study gives an excellent scattering of nuclei and metaphase
plates in one layer as well as a good spreading of chromosomes. It may be
applied to all stages of human oocytes in IVF-ET programs. This method can also
be useful in pre-embryo biopsy and in secondhand quality control for an IVF-ET program.
This may help resolve the present limitations of oocyte chromosome preparation and
analysis, and therefore lead to further improvements in germ cell cytogenetics.