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Amplification of VNTR Locus D1S80 in DNA Recovered from Dried Bloodstains : D1S80 locus의 PCR 증폭법을 이용한 혈흔감정
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Jung Bin | - |
dc.date.accessioned | 2009-08-10T03:34:02Z | - |
dc.date.available | 2009-08-10T03:34:02Z | - |
dc.date.issued | 1991-12 | - |
dc.identifier.citation | Seoul J Med, Vol.32 No.4, pp. 217-221 | - |
dc.identifier.issn | 0582-6802 | - |
dc.identifier.uri | https://hdl.handle.net/10371/6759 | - |
dc.description.abstract | As a method of bloodstain testing for the identification of individuals, DIS
80 locus was analyzed in bloodstains using amplified fragment length polymorphism (AMP-FLP) analysis technique. Dried blood samples were prepared on sections of cotton shirt and glass plate with 10-111 aliquots of blood drawn from two individuals, to simulate bloodstain evidence found at crime scenes. After aging at room temperature for 1 month, 3 months and 11 months, DNA was isolated and amplified with primers of DlS80 locus, followed by acrylamide gel electrophoresis and ethidium bromide staining. The amount of DNA recovered from a 10-111 dried blood aliquot was approximately 440 ng, regardless of the dried pattern or the aging. In quality, the DNA became degraded as aging was extended. The AMP-FLP profile of the bloodstain was identical to that of the peripheral blood, which was not affected by the dried pattern or the aging. | - |
dc.language.iso | en | - |
dc.publisher | Seoul National University College of Medicine | - |
dc.subject | PCR | - |
dc.subject | VNTR | - |
dc.subject | D1S80 | - |
dc.subject | Bloodstain | - |
dc.title | Amplification of VNTR Locus D1S80 in DNA Recovered from Dried Bloodstains | - |
dc.title.alternative | D1S80 locus의 PCR 증폭법을 이용한 혈흔감정 | - |
dc.type | SNU Journal | - |
dc.contributor.AlternativeAuthor | 이정빈 | - |
dc.citation.journaltitle | 서울 의대 잡지 | - |
dc.citation.journaltitle | 서울 의대 학술지 | - |
dc.citation.journaltitle | Seoul Journal of Medicine | - |
dc.citation.endpage | 221 | - |
dc.citation.number | 4 | - |
dc.citation.pages | 217-221 | - |
dc.citation.startpage | 217 | - |
dc.citation.volume | 32 | - |
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