Browse

Effects of Higenamine on the Contractility of Aorta and Taenia Coli in Guinea-piqs
Higenamine이 기니피그 대동맥 및 결장뉴 평활근의 수축성에 미치는 영향

Cited 0 time in Web of Science Cited 0 time in Scopus
Authors
Suk, Sang Bok; Ko, Kwang Wook; Lee, Sang Jin; Hwang, Sang-Ik; Kim, Ki-Whan
Issue Date
1991-12
Publisher
Seoul National University College of Medicine
Citation
Seoul J Med, Vol.32 No.4, pp. 223-238
Keywords
HigenamineAortaTaenia coliNorepinephrine-induced contractionElectrical field stimulationK-contractureSpontaneous contraction
Abstract
Higenamine isolated from Aconiti tuber is known to have positive inotropic
action through the adrenergic ~-receptors. However, the effects of higenamine on the
vascular smooth muscle and taenia coli have not yet been elucidated. The actions of
higenamine were investigated on the activated aortic strips of guinea-pigs and rabbits
by norepinephrine, high K-Tyrode's solution and electrical field stimulation, and also
on the spontaneous contractions of guinea-pig taenia coli. Electrical activities and mechanical
contractions were simultaneously recorded using the conventional suction-electrode
method and single sucrose gap technique. All experiments were performed in
tris-buffered Tyrode's solution which was aerated with 100% O2 and kept at 35°C.
Higenamine suppressed dose-dependently the norepinephrine-induced contraction.
Propranolol potently antagonized the inhibitory effects of higenamine on rabbit aorta,
while less potently on guinea-pig aorta. Higenamine seemed to suppress profoundly
the component of intracellular Ca2+ mobilization in the contraction curve of norepinephrine
in guinea-pig aorta. Higenamine did not seem to suppress the component of
Ca2 + influx through the potential-sensitive Ca2 + channel in K-contracture of guinea-pig
aorta. Higenamine suppressed both passive resting tension and active tension (phasic
contraction) driven by electrical field stimulation (A.C. 60 Hz, 5-7 V/cm, duration 10
sec, interval 3 min) in guinea-pig aorta. Propranolol also rapidly suppressed both passive
resting and active tensions. Higenamine dose-dependently suppressed the frequency
and amplitude of spontaneous contraction, resulting in complete abolishment of it above
10-6 M concentration in guinea-pig taenia coli. Propranolol almost completely antagonized
its effect. Higenamine reduced dose-dependently both burst frequency and spike
frequency, and depolarized membrane potential in guinea-pig taenia coli. Propranolol
almost completely blocked its effect.
In conclusion the inhibitory action of higenamine to the norepinephrine-induced contraction
might be produced by the depression of intracellular Ca2+ mobilization and
Ca2+-influx through ~-adrenoceptors in guinea-pig aorta. The Ca2+-influx through the
potential-sensitive Ca2+ channel might not be suppressed by higenamine in guinea-pig
aorta. The inhibitory effects of higenamine on the spontaneous contractions of guineapig
taenia coli result from the decrease of burst frequency and spike frequency through
the ~-adrenoceptors.
ISSN
0582-6802
Language
English
URI
https://hdl.handle.net/10371/6760
Files in This Item:
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 32 No.4 (1991)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse