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Real-time in vivo monitoring of viable stem cells implanted on biocompatible scaffolds

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dc.contributor.authorHwang, Do Won-
dc.contributor.authorJang, Sung June-
dc.contributor.authorKim, Yun Hui-
dc.contributor.authorKim, Hyun Joo-
dc.contributor.authorShim, In Kyong-
dc.contributor.authorJeong, Jae Min-
dc.contributor.authorChung, June-Key-
dc.contributor.authorLee, Myung Chul-
dc.contributor.authorLee, Seung Jin-
dc.contributor.authorKim, Seung U-
dc.contributor.authorKim, Soonhag-
dc.contributor.authorLee, Dong Soo-
dc.date.accessioned2010-06-24T05:20:54Z-
dc.date.available2010-06-24T05:20:54Z-
dc.date.issued2008-04-26-
dc.identifier.citationEur J Nucl Med Mol Imaging. 2008;35(10):1887-98en
dc.identifier.issn1619-7070 (Print)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18437378-
dc.identifier.urihttp://www.springerlink.com/content/b108181wx4m14852/fulltext.pdf-
dc.identifier.urihttps://hdl.handle.net/10371/67780-
dc.description.abstractPURPOSE: Three-dimensional fibrous scaffolds provide an environment that enhances transplanted stem cell survival in vivo and facilitates imaging their localization, viability, and growth in vivo. To assess transplanted stem cell viability on biocompatible polymer scaffolds in vivo, we developed in vivo imaging systems for evaluation of implanted viable neural stem cells (NSC) and mesenchymal stem cells (MSC) on scaffolds using luciferase or sodium/iodide symporter (NIS) genes. METHODS: Firefly luciferase stably expressing-C6 cell was established (C6-Fluc). The human neural stem cell, F3, was infected with adenoviral vector carrying luciferase gene (F3-Fluc) and MSC expressing NIS controlled by ubiquitin C promoter using lentiviral vector was established by treating blasticidine for 2 weeks (MSC-NIS). Chitosan and poly L-lactic acid (PLLA) scaffolds were used for in vivo image. In vivo expression of luciferase and human NIS was examined by bioluminescence image or (99m)Tc-pertechnetate gamma camera image, respectively. The cell/scaffold complex was implanted into subcutaneous or abdominal area of BALB/C nude mouse. For quantitative evaluation of cell viability, regions of interest were drawn on (99m)Tc-pertechnetate scintigraphy by manual. RESULTS: The gradual increase of luciferase activity was observed in C6-Fluc seeded with chitosan according to the increase in the number of cells. C6-Fluc/chitosan complex subcutaneously implanted into nude mice showed longitudinal bioluminescence image until 34 days. Luciferase image of abdominal-injected C6-Fluc/PLLA complex was saturated in only 14 days, showing great cell growth due to abundant nutrients. F3 cells showed well-incorporated pattern with fibrous chitosan scaffold using scanning electron microscopy. F3 infected with Ad-Fluc showed >100-fold higher luciferase activity than luciferase activity in F3. Cell-number-dependent increase of luciferase activity was shown in F3-Fluc seeded on chitosan. F3-Fluc incorporation into chitosan after abdominal injection was clearly visible on bioluminescence image up to 11 days. Radionuclide imaging showed higher uptake by MSC-NIS on PLLA scaffolds than by MSC-NIS not seeded on a scaffold. Quantitative data showed significantly better survival of MSC-NIS on PLLA scaffolds than without scaffold at 72 h post-implantation, which concurred with histologic findings. CONCLUSION: These results suggest that NSC-Fluc and MSC-NIS cells incorporated within polymer scaffolds can be monitored on a long-term basis by serial in vivo imaging. We believe that a biocompatible scaffold-based imaging system could be used to assess stem cell viabilities in a non-invasive way to aid the development of regenerative therapeutics.en
dc.language.isoen-
dc.publisherSpringer Verlagen
dc.subjectAnimalsen
dc.subjectAutoradiography/*methodsen
dc.subjectCell Culture Techniques/*methodsen
dc.subjectCell Lineen
dc.subjectComputer Systemsen
dc.subjectLuminescent Measurements/*methodsen
dc.subjectMiceen
dc.subjectMice, Inbred BALB Cen
dc.subjectMice, Nudeen
dc.subject*Molecular Probe Techniquesen
dc.subjectStem Cell Transplantation/*methodsen
dc.subjectStem Cells/*cytology/*radionuclide imagingen
dc.subjectTissue Engineering/*methodsen
dc.subjectWhole Body Imaging/*methodsen
dc.titleReal-time in vivo monitoring of viable stem cells implanted on biocompatible scaffoldsen
dc.typeArticleen
dc.contributor.AlternativeAuthor황도원-
dc.contributor.AlternativeAuthor장성준-
dc.contributor.AlternativeAuthor김윤휘-
dc.contributor.AlternativeAuthor김현주-
dc.contributor.AlternativeAuthor심인경-
dc.contributor.AlternativeAuthor정재민-
dc.contributor.AlternativeAuthor정준기-
dc.contributor.AlternativeAuthor이명철-
dc.contributor.AlternativeAuthor이승진-
dc.contributor.AlternativeAuthor김승유-
dc.contributor.AlternativeAuthor김순학-
dc.contributor.AlternativeAuthor이동수-
dc.identifier.doi10.1007/s00259-008-0751-z-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Nuclear Medicine (핵의학전공)Journal Papers (저널논문_핵의학전공)
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