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Kinetic modeling of 3'-deoxy-3'-18F-fluorothymidine for quantitative cell proliferation imaging in subcutaneous tumor models in mice
DC Field | Value | Language |
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dc.contributor.author | Kim, Su Jin | - |
dc.contributor.author | Lee, Jae Sung | - |
dc.contributor.author | Im, Ki Chum | - |
dc.contributor.author | Kim, Seog-Young | - |
dc.contributor.author | Park, Soo-Ah | - |
dc.contributor.author | Lee, Seung Jin | - |
dc.contributor.author | Oh, Seung Jun | - |
dc.contributor.author | Lee, Dong Soo | - |
dc.contributor.author | Moon, Dae Hyuk | - |
dc.date.accessioned | 2010-06-25T05:14:59Z | - |
dc.date.available | 2010-06-25T05:14:59Z | - |
dc.date.issued | 2008-11-11 | - |
dc.identifier.citation | J Nucl Med. 2008;49(12):2057-2066 | en |
dc.identifier.issn | 0161-5505 (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18997037 | - |
dc.identifier.uri | http://jnm.snmjournals.org/cgi/reprint/49/12/2057.pdf | - |
dc.identifier.uri | https://hdl.handle.net/10371/67792 | - |
dc.description.abstract | 3'-Deoxy-3'-(18)F-fluorothymidine ((18)F-FLT) is a thymidine analog that was developed for measuring tumor proliferation with PET. The aim of this study was to establish a kinetic modeling analysis method for quantitative (18)F-FLT PET studies in subcutaneous tumor models in mice. METHODS: To explore the validity of an image-derived left ventricular input function, we measured equilibrium constants for plasma and whole blood and metabolite fractions in blood after (18)F-FLT injection. In parallel, dynamic (18)F-FLT PET scans were acquired in 24 mice with a small-animal dedicated PET scanner to compare arterial blood activities obtained by PET and blood sampling. We then investigated kinetic models for (18)F-FLT in human epithelial carcinoma (A431) and Lewis lung carcinoma tumor models in mice. Three-compartment models with reversible phosphorylation (k(4) not equal 0, 3C5P) and irreversible phosphorylation (k(4) = 0, 3C4P) and a 2-compartment model (2C3P) were examined. The Akaike information criterion and F statistics were used to select the best model for the dataset. Gjedde-Patlak graphic analysis was performed, and standardized uptake values in the last frame were calculated for comparison purposes. In addition, quantitative PET parameters were compared with Ki-67 immunostaining results. RESULTS: (18)F-FLT equilibrated rapidly (within 30 s) between plasma and whole blood, and metabolite fractions were negligible during PET scans. A high correlation between arterial blood sampling and PET data was observed. For 120-min dynamic PET data, the 3C5P model best described tissue time-activity curves for tumor regions. The net influx of (18)F-FLT (K(FLT)) and k(3) obtained with this model showed reasonable intersubject variability and discrimination ability for tumor models with different proliferation properties. The K(FLT) obtained from the 60- or 90-min data correlated well with that obtained from the 120-min data as well as with the Ki-67 results. CONCLUSION: The image-derived arterial input function was found to be feasible for kinetic modeling studies of (18)F-FLT PET in mice, and kinetic modeling analysis with an adequate compartment model provided reliable kinetic parameters for measuring tumor proliferation. | en |
dc.language.iso | en | - |
dc.publisher | The Society of Nuclear Medicine Inc | en |
dc.subject | Animals | en |
dc.subject | Cell Line, Tumor | en |
dc.subject | Cell Proliferation | en |
dc.subject | Computer Simulation | en |
dc.subject | Dideoxynucleosides/*diagnostic use/*pharmacokinetics | en |
dc.subject | Image Interpretation, Computer-Assisted/methods | en |
dc.subject | Kinetics | en |
dc.subject | Metabolic Clearance Rate | en |
dc.subject | Mice | en |
dc.subject | Mice, Inbred BALB C | en |
dc.subject | Mice, Inbred C57BL | en |
dc.subject | Mice, Nude | en |
dc.subject | Radiopharmaceuticals/diagnostic use/pharmacokinetics | en |
dc.subject | Skin/*metabolism/*radionuclide imaging | en |
dc.subject | Skin Neoplasms/*metabolism/*radionuclide imaging | en |
dc.subject | Models, Biological | - |
dc.title | Kinetic modeling of 3'-deoxy-3'-18F-fluorothymidine for quantitative cell proliferation imaging in subcutaneous tumor models in mice | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 김수진 | - |
dc.contributor.AlternativeAuthor | 이재성 | - |
dc.contributor.AlternativeAuthor | 임기첨 | - |
dc.contributor.AlternativeAuthor | 김석영 | - |
dc.contributor.AlternativeAuthor | 박수아 | - |
dc.contributor.AlternativeAuthor | 이승진 | - |
dc.contributor.AlternativeAuthor | 오승준 | - |
dc.contributor.AlternativeAuthor | 이동수 | - |
dc.contributor.AlternativeAuthor | 문대혁 | - |
dc.identifier.doi | 10.2967/jnumed.108.053215 | - |
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