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TGFbeta mediates activation of transglutaminase 2 in response to oxidative stress that leads to protein aggregation

Cited 49 time in Web of Science Cited 53 time in Scopus
Authors
Shin, Dong-Myung; Jeon, Ju-Hong; Kim, Chai-Wan; Cho, Sung-Yup; Lee, Hye-Jin; Jang, Gi-Yong; Jeong, Eui Man; Lee, Dong-Sup; Kang, Ja-Heon; Melino, Gerry; Park, Sang-Chul; Kim, In-Gyu
Issue Date
2008-03-21
Publisher
Federation of American Society of Experimental Biology (FASEB)
Citation
FASEB Journal. 22(7), 2498-2507
Keywords
Calcium/*metabolismCell LineEnzyme Activation/drug effectsEpithelial Cells/drug effects/enzymologyGTP-Binding Proteins/drug effects/*metabolismHumansLens, Crystalline/drug effects/enzymologyOxidative Stress/drug effects/*physiologyTransfectionTransforming Growth Factor beta/*pharmacologyTransglutaminases/drug effects/*metabolism
Abstract
Transglutaminase 2 (TGase2) is a ubiquitously expressed enzyme that catalyzes irreversible post-translational modification of protein, forming cross-linked protein aggregates. We previously reported that intracellular TGase2 is activated by oxidative stress. To elucidate the functional role of TGase2 activation in cells under the oxidatively stressed condition, we identified the mediator that activates TGase2. In this study, we showed that low levels of oxidative stress trigger the release of TGFbeta, which subsequently activates TGase2 through the nuclear translocation of Smad3. Analysis of substrate proteins reveals that TGase2-mediated protein modification results in a decrease of protein solubility and a collapse of intermediate filament network, which leads to aggregation of proteins. We confirm these results using lens tissues from TGase2-deficient mice. Among several antioxidants tried, only N-acetylcysteine effectively inhibits TGFbeta-mediated activation of TGase2. These results indicate that TGFbeta mediates oxidative stress-induced protein aggregation through activation of TGase2 and suggest that the formation of protein aggregation may not be a passive process of self-assembly of oxidatively damaged proteins but may be an active cellular response to oxidative stress. Therefore, TGFbeta-TGase2 pathway may have implications for both the pathogenesis of age-related degenerative diseases and the development of pharmaceutics.
ISSN
1530-6860 (Electronic)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18353867

http://www.fasebj.org/cgi/reprint/22/7/2498.pdf

https://hdl.handle.net/10371/67793
DOI
https://doi.org/10.1096/fj.07-095455
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College of Medicine/School of Medicine (의과대학/대학원)Anatomy (해부학전공)Journal Papers (저널논문_해부학전공)
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