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Human neural crest stem cells transplanted in rat penile corpus cavernosum to repair erectile dysfunction

Cited 30 time in Web of Science Cited 36 time in Scopus
Authors
Song, Yun Seob; Lee, Hong Jun; Park, In Ho; Lim, Ik Sung; Ku, Ja Hyeon; Kim, Seung U
Issue Date
2008-02-21
Publisher
Wiley-Blackwell
Citation
BJU Int. 2008; 102(2): 220-224
Keywords
AnimalsCells, CulturedEndothelial Cells/physiologyFeasibility StudiesHumansImpotence, Vasculogenic/physiopathology/*surgeryMaleMyocytes, Smooth Muscle/*physiologyNeural Crest/*transplantationPenile Erection/*physiologyPenis/physiopathology/*surgeryRatsReverse Transcriptase Polymerase Chain ReactionStem Cell Transplantation/*methods
Abstract
OBJECTIVE: To investigate the feasibility of applying neural crest stem cells (NCSCs), with multipotent capacity, to repair injury in the penile cavernosum, the HNC10.K10 (K10) immortalized NCSC line was transplanted into the penile cavernosum of adult rats, as one of the causes of erectile dysfunction is damaged penile cavernous smooth muscle cells and sinus endothelial cells. MATERIALS AND METHODS: The K10 human NCSC line was generated via transfection of primary cultured NCSC with a retroviral vector encoding v-myc. K10 NCSCs were transplanted into the cavernosum of adult rats. The expression of cell type-specific markers for endothelial cells (CD31 and von Willebrand factor), and specific markers for smooth muscle cells (smooth muscle cell actin, calponin, and desmin) was determined immunohistochemically in the penile cavernosum of rats 2 weeks after transplantation. RESULTS: In the rat cavernosum, transplanted K10 NCSCs identified by human nuclear antigen labelling expressed cell type-specific markers for endothelial cells (CD31 and von Willebrand factor), and specific markers for smooth muscle cells (smooth muscle cell actin, calponin, and desmin) 2 weeks after transplantation. Human NCSCs transplanted into the rat penile corpus cavernosum differentiated into endothelial cells or smooth muscle cells, as shown by their expression of cell type-specific markers for the cell types. CONCLUSION: It appears that NCSCs are an ideal cell source for reconstructing endothelial and smooth muscle cells in the corpus cavernosum in cell therapy for patients with erectile dysfunction.
ISSN
1464-410X (Electronic)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18284412

http://hdl.handle.net/10371/67936
DOI
https://doi.org/10.1111/j.1464-410X.2008.07469.x
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College of Medicine/School of Medicine (의과대학/대학원)Urology (비뇨기과학전공)Journal Papers (저널논문_비뇨기과학전공)
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