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Negative feedback regulation of Aurora-A via phosphorylation of Fas-associated factor-1
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jang, Moon-Sun | - |
dc.contributor.author | Sul, Jee-Won | - |
dc.contributor.author | Choi, Byung-Jung | - |
dc.contributor.author | Lee, Su-Jin | - |
dc.contributor.author | Suh, Jee-Hee | - |
dc.contributor.author | Kim, Nam-Soon | - |
dc.contributor.author | Kim, Woo Ho | - |
dc.contributor.author | Lim, Dae-Sik | - |
dc.contributor.author | Lee, Chang-Woo | - |
dc.contributor.author | Kim, Eunhee | - |
dc.date.accessioned | 2010-06-30T06:17:10Z | - |
dc.date.available | 2010-06-30T06:17:10Z | - |
dc.date.issued | 2008-09-16 | - |
dc.identifier.citation | J Biol Chem. 283(47), 32344-32351 | en |
dc.identifier.issn | 0021-9258 (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18790738 | - |
dc.identifier.uri | http://www.jbc.org/content/283/47/32344.full.pdf | - |
dc.identifier.uri | https://hdl.handle.net/10371/68050 | - |
dc.description.abstract | This study reports that Aurora-A (Aur-A) phosphorylates Fas-associated factor-1 (FAF1) at Ser-289 and Ser-291. Forced expression of a FAF1 mutant mimicking phosphorylation at Ser-289 and Ser-291 (FAF1 DD), but not phosphorylation-deficient FAF1 (FAF1 AA), reduced Aur-A expression. However, transfection of FAF1 DD failed to reduce Aur-A expression in the presence of MG132 and MG115, indicating that this decrease is proteasome-mediated. Additionally, transfection of FAF1 DD suppressed the expression of Aur-A in ts20-BALB cells lacking E1 ubiquitin (Ub) activating enzyme activity at restrictive temperatures and also reduced the expression of Aur-A S51D, a mutant resistant to Ub-dependent degradation. Our data indicate that phosphorylated FAF1 mediates the ubiquitin-independent, proteasome-dependent degradation of Aur-A. Overexpression of FAF1 DD blocked Aur-A-induced centrosome amplification and accumulated cells in G(2)/M phase, representing cellular phenotypes consistent with the anticipated loss of Aur-A. Collectively, our findings support the negative feedback regulation of Aur-A via phosphorylation of the death-promoting protein, FAF1, and disclose the presence of molecular cross-talk between constituents of the cell cycle and cell death machinery. | en |
dc.language.iso | en | en |
dc.publisher | American Society for Biochemistry and Molecular Biology | en |
dc.subject | Adaptor Proteins, Signal Transducing/*metabolism | en |
dc.subject | Animals | en |
dc.subject | Carrier Proteins/*metabolism | en |
dc.subject | Cell Cycle | en |
dc.subject | Feedback, Biochemical | en |
dc.subject | Hela Cells | en |
dc.subject | Humans | en |
dc.subject | Mice | en |
dc.subject | Mice, Inbred BALB C | en |
dc.subject | Phosphorylation | en |
dc.subject | Proteasome Endopeptidase Complex/metabolism | en |
dc.subject | Protein-Serine-Threonine Kinases/*biosynthesis/physiology | en |
dc.subject | RNA Interference | en |
dc.subject | Gene Expression Regulation, Enzymologic | - |
dc.title | Negative feedback regulation of Aurora-A via phosphorylation of Fas-associated factor-1 | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 장문선 | - |
dc.contributor.AlternativeAuthor | 설지원 | - |
dc.contributor.AlternativeAuthor | 최병중 | - |
dc.contributor.AlternativeAuthor | 이수진 | - |
dc.contributor.AlternativeAuthor | 서지희 | - |
dc.contributor.AlternativeAuthor | 김남순 | - |
dc.contributor.AlternativeAuthor | 김우호 | - |
dc.contributor.AlternativeAuthor | 임대식 | - |
dc.contributor.AlternativeAuthor | 이창우 | - |
dc.contributor.AlternativeAuthor | 김은희 | - |
dc.identifier.doi | 10.1074/jbc.M804199200 | - |
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