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Decreased expression of angiogenin in the eutopic endometrium from women with advanced stage endometriosis

Cited 4 time in Web of Science Cited 4 time in Scopus
Authors
Kim, Sung Hoon; Choi, Young Min; Chae, Heen Dong; Kim, Chung Hoon; Kang, Byung Moon
Issue Date
2008-10-29
Publisher
Korean Academy of Medical Sciences
Citation
J Korean Med Sci. 2008; 23(5): 802-807
Keywords
AdultEndometriosis/*metabolism/pathologyEndometrium/*metabolismFemaleFertility*Gene Expression RegulationHumansImmunohistochemistry/methodsMenstrual CycleModels, BiologicalRNA, Messenger/metabolismReverse Transcriptase Polymerase Chain ReactionRibonuclease, Pancreatic/*biosynthesis
Abstract
Angiogenin, a potent inducer of angiogenesis, is expressed in human endometrium. This study was performed to compare the expression of angiogenin mRNA level in the eutopic endometrium from women with and without endometriosis. Thirty-two women with advanced stage endometriosis and 29 control women were recruited. Following isolation of total RNA from endometrial tissue and reverse transcription, cDNA samples were amplified by real time polymerase chain reaction to quantify the expression of angiogenin genes. In selected patients, immunohistochemical staining was utilized to localize the area of angiogenin expression. Angiogenin mRNA level was significantly lower in the endometriosis group than in the control group during the secretory phase, especially the mid-secretory phase, and the decline was observed mainly in the women who presented with infertility. Within the endometriosis group, angiogenin mRNA levels did not differ between the proliferative and secretory phases, but, in the control group, the level in the secretory phase was higher than that during the proliferative phase. Immunohistochemistry showed that the glandular epithelial cell layer was decorated positively in both groups. These findings suggest that the relative deficiency of angiogenin expression in the secretory endometrium could impair implantation in women with advanced stage endometriosis.
ISSN
1011-8934 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18955785

http://synapse.koreamed.org/Synapse/Data/PDFData/0063JKMS/jkms-23-802.pdf

http://hdl.handle.net/10371/68061
DOI
https://doi.org/10.3346/jkms.2008.23.5.802
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College of Medicine/School of Medicine (의과대학/대학원)Obstetrics & Gynecology (산부인과전공)Journal Papers (저널논문_산부인과학전공)
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