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The effects of dopamine receptor agonists on BK Ca channels and signal transduction mechanism in corpus cavernosal smooth muscle cells

Cited 6 time in Web of Science Cited 7 time in Scopus
Authors
Han, D H; Chae, M R; So, I; Park, J K; Lee, S W
Issue Date
2007-12-21
Publisher
Nature Publishing Group
Citation
Int J Impot Res. 2008; 20(1): 53-59
Keywords
Cell Culture TechniquesDopamine Agonists/*pharmacologyHumansLarge-Conductance Calcium-Activated Potassium Channels/drugeffects/*metabolismMaleMuscle, Smooth, Vascular/*drug effectsPenile Erection/*physiologySignal Transduction/*drug effects
Abstract
In this study, we investigated the effect of dopamine receptor agonists on potassium channels' activity and their signal transduction pathway in corporal smooth muscle cells. We used cultured human corporal smooth muscle cells. The whole cell and cell-attached configuration of the patch-clamp technique were used for electrophysiological recordings, and enzyme immunoassay was used for measuring cyclic AMP (cAMP) and cyclic GMP levels. Extracellular application of 10 microM dopamine and apomorphine significantly increased whole-cell K(+) currents by 283.5+/-55.7% (at +60 mV; n=12, P<0.001), 292.4+/-58.8.0% (at +60 mV; n=9, P<0.005), respectively. We confirmed that the increase in whole-cell currents was mainly due to activation of the tetraethylammonium-sensitive large conductance Ca(2+)-activated K(+) channels (BK(Ca) channels). Enzyme immunoassay indicated that dopamine and apomorphine stimulates cAMP levels in corporal smooth muscle cells in a concentration-dependent fashion. The activation of BK(Ca) channels by dopamine receptor agonists in corporal smooth muscle cells might be one of the mechanisms in inducing penile erection.
ISSN
1476-5489 (Electronic)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18094703

http://www.nature.com/ijir/journal/v20/n1/pdf/3901623a.pdf

http://hdl.handle.net/10371/68129
DOI
https://doi.org/10.1038/sj.ijir.3901623
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Physiology (생리학교실)Journal Papers (저널논문_생리학교실)
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