S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Neurology (신경과학교실) Journal Papers (저널논문_신경과학교실)
Na(+)/Ca(2+) exchanger 2 is neuroprotective by exporting Ca(2+) during a transient focal cerebral ischemia in the mouse
- Jeon, Daejong; Chu, Kon; Jung, Keun-Hwa; Kim, Manho; Yoon, Byung-Woo; Lee, C Justin; Oh, Uhtaek; Shin, Hee-Sup
- Issue Date
- Cell Calcium. 43 (2008) 482-491
- Animals; Biological Transport; Brain/physiopathology; Calcium/*metabolism; Cell Survival; Cerebral Infarction/pathology; Ischemic Attack, Transient/*metabolism/pathology/physiopathology; Membrane Potentials; Mice; Mice, Knockout; Neurons/pathology; Neuroprotective Agents/metabolism; Patch-Clamp Techniques; Sodium-Calcium Exchanger/genetics/metabolism/*physiology
- Na(+)/Ca(2+) exchanger (NCX), by mediating Na(+) and Ca(2+) fluxes bi-directionally, assumes a role in controlling the Ca(2+) homeostasis in the ischemic brain. It has been suggested that the three isoforms of NCX (NCX1, 2 and 3) may be differentially involved in permanent cerebral ischemia. However, the role of NCX2 has not been defined in ischemic reperfusion injury after a transient focal cerebral ischemia. Furthermore, it is not known whether NCX2 imports or exports intracellular Ca(2+) ([Ca(2+)](i)) following ischemia and reperfusion. To define the role of NCX2 in ischemia and reperfusion, we examined mice lacking NCX2, in vivo and in vitro. After an in vitro ischemia, a significantly slower recovery in population spike amplitudes, a sustained elevation of [Ca(2+)](i) and an increased membrane depolarization were developed in the NCX2-deficient hippocampus. Moreover, a transient focal cerebral ischemia in vivo produced a larger infarction and more cell death in the NCX2-deficient mouse brain. In particular, in the wild type brain, NCX2-expressing neurons were largely spared from cell death after ischemia. Our results suggest that NCX2 exports Ca(2+) in ischemia and thus protects neuronal cells from death by reducing [Ca(2+)](i) in the adult mouse brain.
- 0143-4160 (Print)
- Files in This Item: There are no files associated with this item.