Browse

Glutathione peroxidase 3 mediates the antioxidant effect of peroxisome proliferator-activated receptor gamma in human skeletal muscle cells

DC Field Value Language
dc.contributor.authorChung, Sung Soo-
dc.contributor.authorKim, Min-
dc.contributor.authorYoun, Byoung-Soo-
dc.contributor.authorLee, Nam Seok-
dc.contributor.authorPark, Ji Woo-
dc.contributor.authorLee, In Kyu-
dc.contributor.authorLee, Yun Sok-
dc.contributor.authorKim, Jae Bum-
dc.contributor.authorCho, Young Min-
dc.contributor.authorLee, Hong Kyu-
dc.contributor.authorPark, Kyong Soo-
dc.date.accessioned2010-07-01T23:26:16Z-
dc.date.available2010-07-01T23:26:16Z-
dc.date.issued2008-10-22-
dc.identifier.citationMol Cell Biol. 29(1):20-30en
dc.identifier.issn1098-5549 (Electronic)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18936159-
dc.identifier.urihttp://mcb.asm.org/cgi/reprint/29/1/20.pdf-
dc.identifier.urihttp://hdl.handle.net/10371/68182-
dc.description.abstractOxidative stress plays an important role in the pathogenesis of insulin resistance and type 2 diabetes mellitus and in diabetic vascular complications. Thiazolidinediones (TZDs), a class of peroxisome proliferator-activated receptor gamma (PPARgamma) agonists, improve insulin sensitivity and are currently used for the treatment of type 2 diabetes mellitus. Here, we show that TZD prevents oxidative stress-induced insulin resistance in human skeletal muscle cells, as indicated by the increase in insulin-stimulated glucose uptake and insulin signaling. Importantly, TZD-mediated activation of PPARgamma induces gene expression of glutathione peroxidase 3 (GPx3), which reduces extracellular H(2)O(2) levels causing insulin resistance in skeletal muscle cells. Inhibition of GPx3 expression prevents the antioxidant effects of TZDs on insulin action in oxidative stress-induced insulin-resistant cells, suggesting that GPx3 is required for the regulation of PPARgamma-mediated antioxidant effects. Furthermore, reduced plasma GPx3 levels were found in patients with type 2 diabetes mellitus and in db/db/DIO mice. Collectively, these results suggest that the antioxidant effect of PPARgamma is exclusively mediated by GPx3 and further imply that GPx3 may be a therapeutic target for insulin resistance and diabetes mellitus.en
dc.description.sponsorshipThis study was supported by 21C Frontier Functional Proteomics
Project from the Korean Ministry of Education, Science and Technology and MarineBio21, Ministry of Maritime Affairs and Fisheries,
South Korea.
en
dc.language.isoenen
dc.publisherAmerican Society for Microbiologyen
dc.subjectAnimalsen
dc.subjectAntioxidants/*metabolismen
dc.subjectBiological Transport/drug effectsen
dc.subjectDiabetes Mellitus/enzymologyen
dc.subjectDisease Models, Animalen
dc.subjectExtracellular Space/drug effects/metabolismen
dc.subjectGlucose/metabolismen
dc.subjectGlutathione Peroxidase/*metabolismen
dc.subjectHumansen
dc.subjectHydrogen Peroxide/metabolismen
dc.subjectInsulin/metabolismen
dc.subjectIntracellular Space/drug effects/metabolismen
dc.subjectMiceen
dc.subjectMice, Inbred C57BLen
dc.subjectMuscle Cells/drug effects/*enzymologyen
dc.subjectMuscle, Skeletal/*cytology/*enzymologyen
dc.subjectPPAR gamma/*metabolismen
dc.subjectResponse Elementsen
dc.subjectSignal Transduction/drug effectsen
dc.subjectThiazolidinediones/pharmacologyen
dc.titleGlutathione peroxidase 3 mediates the antioxidant effect of peroxisome proliferator-activated receptor gamma in human skeletal muscle cellsen
dc.typeArticleen
dc.contributor.AlternativeAuthor정성수-
dc.contributor.AlternativeAuthor김민-
dc.contributor.AlternativeAuthor윤병수-
dc.contributor.AlternativeAuthor이남석-
dc.contributor.AlternativeAuthor박지우-
dc.contributor.AlternativeAuthor이인규-
dc.contributor.AlternativeAuthor김재범-
dc.contributor.AlternativeAuthor조영민-
dc.contributor.AlternativeAuthor이홍규-
dc.contributor.AlternativeAuthor박경수-
dc.contributor.AlternativeAuthor이윤석-
dc.identifier.doi10.1128/MCB.00544-08-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
Files in This Item:
There are no files associated with this item.
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse