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Arachidonic acid-induced activation of large-conductance potassium channels and membrane hyperpolarization in mouse B cells
DC Field | Value | Language |
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dc.contributor.author | Zheng, Haifeng | - |
dc.contributor.author | Nam, Joo Hyun | - |
dc.contributor.author | Nguen, Yen Hoang | - |
dc.contributor.author | Kang, Tong Mook | - |
dc.contributor.author | Kim, Tae Jin | - |
dc.contributor.author | Earm, Yung E | - |
dc.contributor.author | Kim, Sung Joon | - |
dc.date.accessioned | 2010-07-02T04:11:25Z | - |
dc.date.available | 2010-07-02T04:11:25Z | - |
dc.date.issued | 2008-01-25 | - |
dc.identifier.citation | Pflugers Arch. 456(5):867-881 | en |
dc.identifier.issn | 0031-6768 (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18214524 | - |
dc.identifier.uri | http://www.springerlink.com/content/at03t94mq9271081/fulltext.pdf | - |
dc.identifier.uri | https://hdl.handle.net/10371/68188 | - |
dc.description.abstract | Lymphocytes express voltage-gated (Kv) and Ca(2+)-activated (IKCa1) K(+) channels. Recently, we found that WEHI-231, an immature B cell line, expresses voltage-independent K(+) channels called large-conductance background K( + ) channels (LK(bg)). Arachidonic acid (AA) has attracted attention because of its potential regulatory roles in the apoptosis of immature B cells. To elucidate the functional targets of AA, we investigated the effects of AA on membrane currents, voltages, and cytoplasmic Ca(2+) concentration ([Ca(2+)](c)) of WEHI-231 and Bal-17 cells that represent immature and mature mouse B cells, respectively. In whole-cell patch clamp, both Kv and IKCa1 were inhibited by AA. On the other hand, AA activated LK(bg) current and non-selective cationic (NSC) current in WEHI-231 while only NSC current in Bal-17. Inside-out patch clamp study showed that AA directly activates LK(bg). AA induced hyperpolarization of WEHI-231 and depolarization of Bal-17 cells, respectively. The selective functional expression of LK(bg) and their activation by AA were also confirmed in the immature B cells (B220(+)/AA4.1(+)) freshly isolated from mouse spleen. In fura-2 spectrofluorimetry, AA induced persistent increase in [Ca(2+)](c) of WEHI-231 cells, which was attenuated by KCl-induced depolarization. In Bal-17 cell, however, AA induced only a transient increase of [Ca(2+)](c). In summary, the novel type of background K(+) channels (LK(bg)) in immature B cells is strongly activated while the other K(+) channels (Kv and IKCa1) commonly expressed in lymphocytes are inhibited by AA. The hyperpolarization and augmentation of Ca(2+) influx by LK(bg) activation might play a role in the response of immature B cells to AA. | en |
dc.description.sponsorship | This work was supported by grant R01-2005-
000-10231-0 from the Korea Science & Engineering Foundation. | en |
dc.language.iso | en | en |
dc.publisher | Springer Verlag | en |
dc.subject | Animals | en |
dc.subject | Arachidonic Acid/metabolism/*pharmacology | en |
dc.subject | Calcium/metabolism | en |
dc.subject | Calcium Signaling/physiology | en |
dc.subject | Cell Line | en |
dc.subject | Membrane Potentials/*drug effects/physiology | en |
dc.subject | Mice | en |
dc.subject | Patch-Clamp Techniques | en |
dc.subject | Potassium Channels/*metabolism | en |
dc.subject | B-Lymphocytes/drug effects/metabolism | - |
dc.title | Arachidonic acid-induced activation of large-conductance potassium channels and membrane hyperpolarization in mouse B cells | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 남주현 | - |
dc.contributor.AlternativeAuthor | 강동묵 | - |
dc.contributor.AlternativeAuthor | 김태진 | - |
dc.contributor.AlternativeAuthor | 엄융의 | - |
dc.contributor.AlternativeAuthor | 김성준 | - |
dc.identifier.doi | 10.1007/s00424-008-0445-1 | - |
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