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Susceptibility of porcine keratocytes to immune-mediated damage in xeno-related rejection
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, M K | - |
dc.contributor.author | Oh, J Y | - |
dc.contributor.author | Lee, H I | - |
dc.contributor.author | Ko, J H | - |
dc.contributor.author | Lee, H J | - |
dc.contributor.author | Lee, J H | - |
dc.contributor.author | Wee, W R | - |
dc.date.accessioned | 2010-07-05 | - |
dc.date.available | 2010-07-05 | - |
dc.date.issued | 2008-04-01 | - |
dc.identifier.citation | Transplant Proc. 40(2):564-569 | en |
dc.identifier.issn | 0041-1345 (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18374130 | - |
dc.identifier.uri | http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6VJ0-4S4SK83-2J-9&_cdi=6080&_user=168665&_orig=search&_coverDate=03%2F31%2F2008&_sk=999599997&view=c&wchp=dGLbVzb-zSkWb&md5=30591250bbf2f3ac76f6c25a0f421d2a&ie=/sdarticle.pdf | - |
dc.identifier.uri | https://hdl.handle.net/10371/68228 | - |
dc.description.abstract | We admixed cultured porcine keratocytes or corneal endothelial cells in the presence of human sera or peripheral blood mononuclear cells (PBMCs) for 4 to 72 hours to investigate their immune-related susceptibilities to xeno-related rejection. We evaluated complement deposition at 48 hours by flow cytometry after staining with the C3 anti-goat cy3 antibody. The inhibition of proliferation of porcine corneal cells by human sera was examined using the 3-[4,5-dimethy/thiazol-2,5-dephenyl tetrazolium bromide (MTT) assay over 24 to 72 hours. The amount of 51chromium (Cr)-release was estimated after a reaction between the porcine cells and human PBMCs for 4 hours. There was greater C3 deposition in keratocytes (60.2%) than in endothelial cells (26.9%; P = .05, Mann-Whitney U test). Both keratocytes and endothelial cells showed significant levels of proliferative inhibition over a period of 72 hours. The number of 51Cr-release cells on interleukin-2 addition was significantly higher among keratocytes (88.0%) than endothelial cells (51.4%) at a 1:100 target:effector ratio (P = .04, Mann-Whitney U test). Our present data suggested that porcine keratocytes might be key target cells in xeno-related rejections when the porcine cornea is transplanted to primates. | en |
dc.language.iso | en | en |
dc.publisher | Elsevier | en |
dc.subject | Animals | en |
dc.subject | Antibody-Dependent Cell Cytotoxicity | en |
dc.subject | Cells, Cultured | en |
dc.subject | Cornea/*cytology/*immunology | en |
dc.subject | Corneal Transplantation/*immunology | en |
dc.subject | DNA Primers | en |
dc.subject | Endothelial Cells/*cytology/*immunology | en |
dc.subject | Endothelins/genetics | en |
dc.subject | Graft Rejection/*immunology/pathology | en |
dc.subject | Humans | en |
dc.subject | Reverse Transcriptase Polymerase Chain Reaction | en |
dc.subject | Swine | en |
dc.subject | Transplantation, Heterologous/*immunology/pathology | en |
dc.subject | Vimentin/genetics | en |
dc.title | Susceptibility of porcine keratocytes to immune-mediated damage in xeno-related rejection | en |
dc.type | Article | en |
dc.identifier.doi | 10.1016/j.transproceed.2007.12.024 | - |
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