S-Space College of Medicine/School of Medicine (의과대학/대학원) Ophthalmology (안과학전공) Journal Papers (저널논문_안과학전공)
The anti-inflammatory and anti-angiogenic role of mesenchymal stem cells in corneal wound healing following chemical injury
- Oh, Joo Youn; Kim, Mee Kum; Shin, Mi Sun; Lee, Hyun Ju; Ko, Jung Hwa; Wee, Won Ryang; Lee, Jin Hak
- Issue Date
- Stem Cells. 2008;26(4):1047-1055
- Animals; Burns, Chemical/pathology/*surgery; Cell Line; Cells, Cultured; Cornea/*injuries/pathology/surgery; Inflammation/pathology/prevention & control/surgery; Male; Mesenchymal Stem Cell Transplantation/*methods; Neovascularization, Pathologic/chemically induced/pathology/*surgery; Rats; Rats, Sprague-Dawley; Wound Healing/*physiology
- To investigate the anti-inflammatory and anti-angiogenic effects of mesenchymal stem cells (MSC) in the chemically burned corneas, we mechanically removed the corneal epithelium of rats after 100% alcohol instillation. The rats were then randomized into four groups: fresh media, conditioned media derived from the MSC culture (MSC-CM), MSC applied topically to the damaged corneas for 2 hours immediately after the injury or MSC-CM applied either once or 3 times per day for 3 consecutive days. Corneal surface was evaluated every week. After 3 weeks, the corneas were stained with the hematoxylin-eosin, and the expression of interleukin (IL)-2, interferon (IFN)-gamma, IL-6, IL-10, transforming growth factor (TGF)-beta1, thrombospondin-1 (TSP-1), matrix metalloproteinase-2 (MMP-2), and vascular endothelial growth factor (VEGF) were analyzed. CD4+ cells were assessed in the corneas. We found that both MSC and three-time applied MSC-CM (1) reduced corneal inflammation and neovascularization, (2) decreased IL-2 and IFN-gamma, although increased IL-10 and TGF-beta1 as well as IL-6, (3) reduced the infiltration of CD4+ cells, and (4) upregulated the expression of TSP-1, although downregulated that of MMP-2. Interestingly, whereas three-time application of MSC-CM was partially effective, transplantation of MSC achieved a better outcome in suppressing corneal inflammation. The results of this study suggest that the anti-inflammatory and anti-angiogenic action of MSC in the chemically burned corneas might be mediated in part through paracrine pathways involving soluble factors such as IL-10, TGF-beta1, IL-6 and TSP-1.
- 1549-4918 (Electronic)
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