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Cytokine secretion by human mesenchymal stem cells cocultured with damaged corneal epithelial cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Oh, Joo Youn | - |
dc.contributor.author | Kim, Mee Kum | - |
dc.contributor.author | Shin, Mi Sun | - |
dc.contributor.author | Wee, Won Ryang | - |
dc.contributor.author | Lee, Jin Hak | - |
dc.date.accessioned | 2010-07-05T03:53:32Z | - |
dc.date.available | 2010-07-05T03:53:32Z | - |
dc.date.issued | 2009-02-19 | - |
dc.identifier.citation | Cytokine. 2009;46(1):100-103 | en |
dc.identifier.issn | 1096-0023 (Electronic) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19223198 | - |
dc.identifier.uri | http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WDF-4VM2K9C-5-5&_cdi=6765&_user=168665&_orig=search&_coverDate=04%2F30%2F2009&_sk=999539998&view=c&wchp=dGLbVzz-zSkzV&md5=b9f8990ca97abf9c24a93cc4acfd9169&ie=/sdarticle.pdf | - |
dc.identifier.uri | https://hdl.handle.net/10371/68260 | - |
dc.description.abstract | We have previously shown that mesenchymal stem cells (MSCs) reduced corneal inflammation and neovascularization in chemically-burned rat corneas in part through paracrine action. In order to identify the molecule(s) involved, we cocultured human MSCs in the following conditions, and examined the alterations in the cytokine secretion profile; (1) human peripheral blood mononuclear cells (hPBMCs), (2) chemically-damaged human corneal epithelial cells (hCECs), (3) hPBMCs/hCECs, (4) hMSCs, (5) hMSCs/hPBMCs, (6) hMSCs/hCECs, (7) hMSCs/hPBMCs/hCECs. We found that the levels of interleukin (IL)-6 and vascular endothelial growth factor (VEGF) by hMSCs markedly increased for hMSC/hCEC cocultures, and this elevation was further remarkable by the addition of hPBMCs. The hMSCs constitutively expressed transforming growth factor (TGF)-beta1, matrix metalloproteinase (MMP)-2, and thrombospondin-1. The secretion of MMP-9 by hCECs was significantly suppressed by hMSCs. Tumor necrosis factor (TNF)-alpha, interferon (IFN)-gamma, and IL-10 were not detectable in any cultures. The data presented herein provide the candidate molecules mediating the MSC-mediated modulation of inflammation and angiogenesis in cornea. | en |
dc.description.sponsorship | This work was supported by the Korean Research Foundation
grant funded by the Korean Government (KRF-E00373 (102028)). | en |
dc.language.iso | en | en |
dc.publisher | Elsevier | en |
dc.subject | Cell Line | en |
dc.subject | Cells, Cultured | en |
dc.subject | Coculture Techniques/methods | en |
dc.subject | Cornea/*cytology/metabolism | en |
dc.subject | Cytokines/*metabolism | en |
dc.subject | Epithelial Cells/*cytology | en |
dc.subject | Humans | en |
dc.subject | Inflammation | en |
dc.subject | Interleukin-6/metabolism | en |
dc.subject | Mesenchymal Stem Cells/*cytology | en |
dc.subject | Models, Biological | en |
dc.subject | Neovascularization, Pathologic | en |
dc.title | Cytokine secretion by human mesenchymal stem cells cocultured with damaged corneal epithelial cells | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 오주연 | - |
dc.contributor.AlternativeAuthor | 김미금 | - |
dc.contributor.AlternativeAuthor | 신미선 | - |
dc.contributor.AlternativeAuthor | 위원량 | - |
dc.contributor.AlternativeAuthor | 이진학 | - |
dc.identifier.doi | 10.1016/j.cyto.2008.12.011 | - |
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