Browse

MCP-1/CCR2 system is involved in high glucose-induced fibronectin and type IV collagen expression in cultured mesangial cells

DC Field Value Language
dc.contributor.authorPark, Jehyun-
dc.contributor.authorRyu, Dong-Ryeol-
dc.contributor.authorLi, Jin Ji-
dc.contributor.authorJung, Dong-Sub-
dc.contributor.authorKwak, Seung-Jae-
dc.contributor.authorLee, Sun Ha-
dc.contributor.authorYoo, Tae-Hyun-
dc.contributor.authorHan, Seung Hyeok-
dc.contributor.authorLee, Jung Eun-
dc.contributor.authorKim, Dong Ki-
dc.contributor.authorMoon, Sung Jin-
dc.contributor.authorKim, Kunhong-
dc.contributor.authorHan, Dae Suk-
dc.contributor.authorKang, Shin-Wook-
dc.date.accessioned2010-07-05T03:55:15Z-
dc.date.available2010-07-05T03:55:15Z-
dc.date.issued2008-06-27-
dc.identifier.citationAm J Physiol Renal Physiol. 295(3): F749-F757en
dc.identifier.issn0363-6127 (Print)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18579703-
dc.identifier.urihttp://ajprenal.physiology.org/cgi/reprint/295/3/F749.pdf-
dc.identifier.urihttps://hdl.handle.net/10371/68261-
dc.description.abstractMonocyte chemoattractant protein-1 (MCP-1) is a potent chemokine that plays an important role in the recruitment of macrophages. Although previous studies have demonstrated the importance of MCP-1 in the pathogenesis of diabetic nephropathy (DN) in terms of inflammation, the role of MCP-1 and its receptor (C-C chemokine receptor 2; CCR2) in extracellular matrix (ECM) accumulation under diabetic conditions has been largely unexplored. This study was undertaken to investigate the functional role of the MCP-1/CCR2 system in high glucose-induced ECM (fibronectin and type IV collagen) protein expression in cultured mesangial cells (MCs). Mouse MCs were exposed to medium containing 5.6 mM glucose (NG), NG+24.4 mM mannitol (NG+M), or 30 mM glucose (HG) with or without mutant MCP-1 (mMCP-1), CCR2 small interfering (si)RNA, or CCR2 inhibitor (RS102895). To examine the relationship between MCP-1 and transforming growth factor (TGF)-beta1, MCs were also treated with TGF-beta1 (2 ng/ml) with or without mMCP-1 or CCR2 siRNA. Transient transfection was performed with Lipofectamine 2000 for 24 h. Cell viability was determined by an MTT assay, mouse and human MCP-1 and TGF-beta1 levels by ELISA, and CCR2 and ECM protein expression by Western blotting. Transfections of mMCP-1 and CCR2 siRNA increased human MCP-1 levels and inhibited CCR2 expression, respectively. HG-induced ECM protein expression and TGF-beta1 levels were significantly attenuated by mMCP-1, CCR2 siRNA, and RS102895 (P < 0.05). MCP-1 directly increased ECM protein expression, and this increase was inhibited by an anti-TGF-beta1 antibody. In addition, TGF-beta1-induced ECM protein expression was significantly abrogated by the inhibition of the MCP-1/CCR2 system (P < 0.05). These results suggest that an interaction between the MCP-1/CCR2 system and TGF-beta1 may contribute to ECM accumulation in DN.en
dc.description.sponsorshipThis work was supported by the BK21 (Brain Korea 21) Project for Medical
Sciences, Yonsei University, a faculty research grant from Yonsei University
College of Medicine for 2007 (6-2007-0174), a Korea Research Foundation
Grant funded by the Korean government (MOEHRD; KRF-2007-331-
E00086), and Korea Science and Engineering Foundation (KOSEF) grants funded by the Korea government (MOST; R01-2007-000-20263-0 and R13-
2002-054-04001-0).
en
dc.language.isoenen
dc.publisherAmerican Physiological Societyen
dc.subjectAnimalsen
dc.subjectCells, Cultureden
dc.subjectChemokine CCL2/genetics/*metabolismen
dc.subjectCollagen Type IV/*metabolismen
dc.subjectDiabetic Nephropathies/metabolismen
dc.subjectFibronectins/*metabolismen
dc.subjectGlucose/metabolismen
dc.subjectHumansen
dc.subjectMesangial Cells/*metabolismen
dc.subjectMiceen
dc.subjectMice, Transgenicen
dc.subjectMutationen
dc.subjectRNA, Small Interfering/geneticsen
dc.subjectReceptors, CCR2/genetics/*metabolismen
dc.subjectTransfectionen
dc.subjectTransforming Growth Factor beta1/metabolismen
dc.titleMCP-1/CCR2 system is involved in high glucose-induced fibronectin and type IV collagen expression in cultured mesangial cellsen
dc.typeArticleen
dc.contributor.AlternativeAuthor박제현-
dc.contributor.AlternativeAuthor류동렬-
dc.contributor.AlternativeAuthor이진지-
dc.contributor.AlternativeAuthor정동섭-
dc.contributor.AlternativeAuthor곽승재-
dc.contributor.AlternativeAuthor이선하-
dc.contributor.AlternativeAuthor유태현-
dc.contributor.AlternativeAuthor한승혁-
dc.contributor.AlternativeAuthor이정은-
dc.contributor.AlternativeAuthor김동기-
dc.contributor.AlternativeAuthor문성진-
dc.contributor.AlternativeAuthor김근홍-
dc.contributor.AlternativeAuthor한대석-
dc.contributor.AlternativeAuthor강신욱-
dc.identifier.doi10.1152/ajprenal.00547.2007-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
Files in This Item:
There are no files associated with this item.
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse