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Expression of neuronal markers in the secondary neurulation of chick embryos

Cited 8 time in Web of Science Cited 9 time in Scopus
Authors
Chung, You-Nam; Lee, Do-Hun; Yang, Hee-Jin; Kim, Seung-Ki; Lee, Yun-Jin; Lee, Myung-Sook; Cho, Byung-Kyu; Kim, Dong-Ho; Wang, Kyu-Chang
Issue Date
2007-09-07
Publisher
Springer Verlag
Citation
Childs Nerv Syst. 24(1):105-110
Keywords
AnimalsBiological Markers/*metabolismCell Adhesion Molecules, Neuronal/metabolismChick EmbryoImmunohistochemistry/methodsNeurofilament Proteins/metabolismNeurons/*metabolismNeurulation/*physiologySynaptophysin/metabolism
Abstract
OBJECTIVE: The goal of our study was to evaluate the expression patterns of neuronal antigens during the process of secondary neurulation. MATERIALS AND METHODS: Chick embryos of Hamburger and Hamilton stages 16, 18, 20, 22, 24, 26, 30, 35, 40, and 45 were harvested, and tail sections were processed for neuronal antigen studies. RESULTS AND CONCLUSIONS: The areas and periods showing positive reactions for each antigen are as follows: neuronal cell adhesion molecule (N-CAM): the secondary neural tube and notochord from stages 18 to 26 and the germinal and mantle layers from stages 30 to 45; synaptophysin: the caudal cell mass, secondary neural tube, and notochord from stages 22 to 26, the germinal and mantle layers from stages 30 to 45, and the marginal layer at the later stages of development; neurofilament-associated protein (3A10): the dorsal white matter, dorsal root ganglion, and scattered cells around the germinal layer from stages 35 to 45; and neuronal nuclear-specific protein (NeuN): the mantle layer at stage 35, which shows decreased reaction at stages 40 and 45; islet-1: no remarkable staining on the caudal cell mass or on the other neural structures at all stages. Our results indicate that neuronal markers of the secondary neurulation in chick embryos have their own chronological patterns of expression. At early stages of secondary neurulation, N-CAM and synaptophysin are thought to modulate the differentiation of structures derived from the caudal cell mass. At later stages, N-CAM, synaptophysin, 3A10, and NeuN seem to be involved in the maturation of the caudal spinal cord.
ISSN
1433-0350 (Electronic)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17805548

http://www.springerlink.com/content/u13hkn2482661012/fulltext.pdf

http://hdl.handle.net/10371/68359
DOI
https://doi.org/10.1007/s00381-007-0440-4
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College of Medicine/School of Medicine (의과대학/대학원)Neurosurgery (신경외과학전공)Journal Papers (저널논문_신경외과학전공)
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