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Effect of niclosamide on the tegumental surface of Haplorchis taichui using scanning electron microscopy

Cited 1 time in Web of Science Cited 2 time in Scopus
Authors
Kumchoo, K.; Wongsawad, C.; Vanittanakom, P.; Chai, J. Y.; Rojanapaibul, A.
Issue Date
2007-06-26
Publisher
Cambridge University Press
Citation
J Helminthol. 81(4), 329-337
Keywords
AnimalsAntinematodal Agents/*pharmacologyHeterophyidae/*drug effects/ultrastructureMicroscopy, Electron, ScanningNiclosamide/*pharmacologyTrematode Infections/prevention & control
Abstract
The effect of niclosamide on the tegument of adult Haplorchis taichui (Trematoda: Heterophyidae) exposed in vitro was observed by scanning electron microscope. Adult worms were incubated in Tyrode's solution containing 0.01, 0.1, 1.0, and 10 microg ml(-1) of niclosamide for 30 min, 1, 6, 12 and 24 h. Control groups were incubated in Tyrode's solution without niclosamide and worms remained active until 24 h. In 0.01 microg ml(-1) of niclosamide, worms showed slightly active movements up to 1 h after incubation, while in 0.1 microg ml(-1) solution a few worms showed only slightly active movements after 30 min. Tegumental changes were determined by scanning electron microscopy. Swelling and blebbing of the tegument were observed on both ventral and dorsal sides. After longer periods, extensive swelling and blebbing of the tegument became more severe and there was a loss of the apical plasma membrane in some regions. Empty spine sockets occurred, and small perforations penetrated the basal lamina, followed by some lesions. Destruction of both surfaces was more pronounced on the posterior compared with the anterior regions.
ISSN
0022-149X (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17588285

http://hdl.handle.net/10371/68480
DOI
https://doi.org/10.1017/S0022149X07381108
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College of Medicine/School of Medicine (의과대학/대학원)Parasitology and Tropical Medicine (기생충학전공)Journal Papers (저널논문_기생충학전공)
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