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Receptor Tyrosine and MAP Kinase are Involved in Effects of H(2)O(2) on Interstitial Cells of Cajal in Murine Intestine

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dc.contributor.authorChoi, Seok-
dc.contributor.authorYeum, Cheol Ho-
dc.contributor.authorKim, Young Dae-
dc.contributor.authorPark, Chan Guk-
dc.contributor.authorKim, Man Yoo-
dc.contributor.authorPark, Jong-Seong-
dc.contributor.authorJeong, Han-Seong-
dc.contributor.authorKim, Byung Joo-
dc.contributor.authorSo, Insuk-
dc.contributor.authorKim, Ki Whan-
dc.contributor.authorJun, Jae Yeoul-
dc.date.accessioned2010-07-08-
dc.date.available2010-07-08-
dc.date.issued2008-07-16-
dc.identifier.citationJ Cell Mol Med. 2008; 141: 257-266en
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18624754-
dc.identifier.urihttps://hdl.handle.net/10371/68490-
dc.description.abstractHydrogen peroxide (H(2)O(2)) is involved in intestinal motility through changes of smooth muscle activity. However, there is no report as to the modulatory effects of H(2)O(2) on interstitial cells of Cajal (ICC). We investigated the H(2)O(2) effects and signal transductions to determine whether the intestinal motility can be modulated through ICC. We performed whole-cell patch clamp in cultured ICC from murine intestine and molecular analyses. H(2)O(2) hyperpolarized the membrane and inhibited pacemaker currents. These effects were inhibited by glibenclamide, an inhibitor of ATP-sensitive K(+) (K(ATP)) channels. The free radical scavenger catalase inhibited the H(2)O(2)-induced effects. MAFP and AACOCF(3) (a cytosolic phospholipase A(2) inhibitors) or SC-560 and NS-398 (a selective COX-1 and 2 inhibitor) or AH6809 (an EP(2) receptor antagonist) inhibited the H(2)O(2)-induced effects. PD98059 (a mitogen activated/ERK-activating protein kinase inhibitor) inhibited the H(2)O(2)-induced effects, though SB-203580 (a p38 MAPK inhibitor) or a JNK inhibitor did not affect. H(2)O(2)-induced effects could not be inhibited by LY-294002 (an inhibitor of PI(3)-kinases), calphostin C (a protein kinase C inhibitor), or SQ-22536 (an adenylate cyclase inhibitor). Adenoviral infection analysis revealed H(2)O(2) stimulated tyrosine kinase activity and AG 1478 (an antagonist of epidermal growth factor receptor tyrosine kinase) inhibited the H(2)O(2)-induced effects. These results suggest H(2)O(2) can modulate ICC pacemaker activity and this occur by the activation of K(ATP) channels through PGE(2) production via receptor tyrosine kinase-dependent MAP kinase activation.en
dc.language.isoenen
dc.publisherWiley-Blackwellen
dc.subjectHydrogen peroxideen
dc.subjectInterstitial cells of Cajalen
dc.subjectPacemaker currentsen
dc.subjectCyclooxygenaseen
dc.subjectReceptor tyrosine kinaseen
dc.subjectMAP kinaseen
dc.titleReceptor Tyrosine and MAP Kinase are Involved in Effects of H(2)O(2) on Interstitial Cells of Cajal in Murine Intestineen
dc.typeArticleen
dc.contributor.AlternativeAuthor최석-
dc.contributor.AlternativeAuthor염철호-
dc.contributor.AlternativeAuthor김영대-
dc.contributor.AlternativeAuthor박찬국-
dc.contributor.AlternativeAuthor김만유-
dc.contributor.AlternativeAuthor박종성-
dc.contributor.AlternativeAuthor정한성-
dc.contributor.AlternativeAuthor김병주-
dc.contributor.AlternativeAuthor소인석-
dc.contributor.AlternativeAuthor김기완-
dc.contributor.AlternativeAuthor전재열-
dc.identifier.doi10.1111/j.1582-4934.2008.00403.x-
dc.identifier.doi10.1111/j.1582-4934.2008.00403.x-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Dept. of Physiology (생리학교실)Journal Papers (저널논문_생리학교실)
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