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Characterization of high affinity neurotensin receptor NTR1 in HL-60 cells and its down regulation during granulocytic differentiation.
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Se-Young | - |
dc.contributor.author | Chae, Hee-Don | - |
dc.contributor.author | Park, Tae-Ju | - |
dc.contributor.author | Ha, Hyunjung | - |
dc.contributor.author | Kim, Kyong-Tai | - |
dc.date.accessioned | 2010-07-12T01:05:28Z | - |
dc.date.available | 2010-07-12T01:05:28Z | - |
dc.date.issued | 1999 | - |
dc.identifier.citation | British Journal of Pharmacology 126, 1050 - 1056 | en |
dc.identifier.issn | 0007-1188 | - |
dc.identifier.uri | https://hdl.handle.net/10371/68572 | - |
dc.description.abstract | We investigated responses to neurotensin in human promyelocytic leukaemia HL-60 cells.
Neurotensin increased the cytosolic calcium concentration ([Ca2+]i) in a concentration-dependent manner and also produced inositol 1,4,5-trisphosphate (InsP3). Among the tested neurotensin analogues, neurotensin 8–13, neuromedin-N, and xenopsin also increased [Ca2+]i, whereas neurotensin 1–11 and neurotensin 1–8 did not elicit detectable responses. SR48692, an antagonist of NTR1 neurotensin receptors, blocked the neurotensin-induced [Ca2+]i increase, whereas levocabastine, which is known as an NTR2 neurotensin receptor antagonist, did not attenuate the neurotensin-evoked effect. The expression of NTR1 neurotensin receptors was confirmed by Northern blot analysis and reverse transcriptase-polymerase chain reaction (RT–PCR). During 1.25% dimethylsulfoxide (DMSO)-triggered granulocytic differentiation of HL-60 cells, the neurotensin-induced [Ca2+]i rise became gradually smaller and completely disappeared 4 days after treatment with DMSO. The mRNA level for neurotensin receptors was also decreased after differentiation. The results show that HL-60 cells express NTR1 neurotensin receptors and suggest that granulocytic differentiation involves transcriptional regulation of the receptors resulting in down-regulation of the neurotensin-induced signalling. | en |
dc.description.sponsorship | This study was supported by grants from the Basic
Science Research Institute Program (Project BSRI-98-4435) from the Ministry of Education. S.Y. Choi was supported by research fund for young scientists from Korea Research Foundation (1998). | en |
dc.language.iso | en | en |
dc.publisher | Nature Publishing Group | en |
dc.subject | Neurotensin | en |
dc.subject | cytosolic Ca2+ | en |
dc.subject | phospholipase C | en |
dc.subject | human hemopoietic cells | en |
dc.subject | di erentiation | en |
dc.title | Characterization of high affinity neurotensin receptor NTR1 in HL-60 cells and its down regulation during granulocytic differentiation. | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 최세영 | - |
dc.contributor.AlternativeAuthor | 채희돈 | - |
dc.contributor.AlternativeAuthor | 박태주 | - |
dc.contributor.AlternativeAuthor | 하현중 | - |
dc.contributor.AlternativeAuthor | 김경태 | - |
dc.identifier.doi | 10.1038/sj.bjp.0702378 | - |
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