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A role of sodium bicarbonate cotransporter(NBC) in HCO3- formation in human salivary gland acinar cells
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- Authors
- Issue Date
- 2008
- Publisher
- The Korean Academy of Oral Biology
- Citation
- International Journal of Oral Biology, 2008;33(4):143-147
- Keywords
- sodium bicarbonate cotransporter (NBC) ; human parotid gland ; pHi recovery ; 4'-diisothio cyanatostilbene-2 ; 2'-disulphonic acid (DIDS) ; acetazolamide
- Abstract
- The sodium bicarbonate cotransporter (NBC) protein is functionally expressed in salivary glands. In this experiment, we examined the role of NBC in HCO3- formation in human parotid gland acinar cells. Intracellular pH (pHi) was measured in 2'-7'-bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF) -loaded cells. Acetazolamide (0.1 mM) and 4,4'-diisothiocyanatostilbene-2,2'-disulphonic acid (DIDS, 0.5 mM) were used as specific inhibitors of carbonic anhydrase and NBC, respectively. The degree of inhibition was assessed by measuring the pHi recovery rate (△pHi/min) after cell acidification using an ammonium prepulse technique. In control experiments, △pHi/min was 1.40 ± 0.06. Treatment of cells with 0.5 mM DIDS or 0.1 mM acetazolamide significantly reduced △pH/min to 1.14 ± 0.14 and 0.74 ± 0.15, respectively. Simultaneous application of DIDS and acetazolamide further reduced △ pHi/min to 0.47 ± 0.10. Therefore, DIDS and acetazolamide reduced △pHi/min by 19 % and 47 %, respectively, while simultaneous application of both DIDS and acetazolamide caused a reduction in △pHi/min of 67 %. These results suggest that in addition to carbonic anhydrase, NBC also partially contributes to HCO3- formation in human parotid gland acinar cells.
- ISSN
- 1226-7155
- Language
- Korean
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