토끼 태자에 형성시킨 구순열상의 치유과정에서 수종 성장인자 분포에 관한 연구

Abstract

성체의 상처 치유과정중 반흔조직 형성에 수종의 성장인자가 관련이 있는 것으로 보고되고 있으나, 태자의 피부상처가 반흔형성없이 치유되는 기전에 관한 성장인자의 역할은 아직 알려져 있지 않다. 따라서 본 연구의 목적은 토끼태자의 상처 치유과정에서 반흔조직의 형성과 관련이 있는 수종 성장인자의 분포를 조사하기 위한 것이다. 뉴질랜드산 흰 토끼를 임신 3기의 중반인 24일째에 자궁절개술을 시행하고 태자에 인위적으로 구순열상을 만든 후, 봉합한 군(봉합군)과 봉합하지 않은 군(비봉합군), 정상대조군(sham operated control group)으로 나누고 이들을 각각 수술후 1, 2, 3, 5, 7일째에 희생시켜 상처치유에 대한 육안관찰 소견과 H&E염색 소견 및 TGF-β1, TGF-β2, PDGF, bFGF의 면역조직화학적 염색 소견을 관찰하여 다음과 같은 결론을 얻었다. 1. 봉합군과 비봉합군에서 전기간동안 염증반응과 반흔조직의 형성 그리고 신생 혈관의 형성 증가는 없었다. 2. 비봉합군의 재생피화가 봉합군에 비해 다소 느렸다. 3. bFGF는 정상대조곤, 봉합군과 비봉합군에서 차이가 없었다. 4. PDGF는 봉합군과 비봉합군에서 1, 2일군에서 증가하였다가 그후 감소하여 정상대조군과 차이가 없었다. 5. TGF-β는 봉합군에서 1, 2일군에서 약간 증가하였다가 그후 감소하여 정상대조군과 차이가 없었다. TGF-β1에 비하여 TGF-β2의 검출양이 많았다.

Recently several growth factors such as TGF-β1, TGF-β2, PDGF, bFGF are known to play an important role in scar formation following adult tissue injury. But there is little known about the role of growth factors tissue healing without scar formation. Therefore the pupose of this study is to investigate the distribution of growth factors which are involved with scar formation in the artificially created lip wound healing of fetuses. The author had undergone hysterotomy and created cleft lip-like defects on fetuses of New Zealand White Rabbit in mid-third trimester (24 days). Fetuses were divided into 3 groups (the repired group, the unrepaired group and the sham-operated control group). At 1, 2, 3, 5, 7 days agter procedure, the repaired, the unrepaired and the control groups were obtained by Caeserean section. After documenting the viability of fetuses, fetuses were photographed to compare size and facial morphology and sectioned for histological examination by H & E stain and spatial and temporal deposition of TGF-β1, TGF-β2, PDGF, bFGF by immunohistochemical method. The findings are summarized as follows. 1. There were lack of inflammation and scar formation and nevascularity in the repaired and the unrepaired group during experimental periods. 2. The reepothelialization of the unrepaired group was slower than that of repaired group. 3. There wereon differences of distribution of bFGF in the control, the repaired and the unrenaired group. 4. PDGF was increased at post-op, first and second day and decreased agter post-op, third day. Eventully, there were no differences in the control, the repaired and the unrepaired group. 5. TGF-β1 and TGF-β2 were slightly increased at post-op, first and second day decreased after post-op, thied day. Eventually there were no differences in the control, the repaired and the unrepaired group. And TGF-β2 is more densely stained than TGF-β1.