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Nuclear transfer by Using a Laser-Assisted Zona Pellucida Piercing Technique in Mice

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Authors
Min, Byung-Moo; Kang, Hoin; Choi, Young-Ju; Sung, Jihye; Park, Sang-Kyu; Roh, Sangho
Issue Date
2007-09
Publisher
Korean Acadamy of Oral Biology
Citation
International Journal of Oral Biology 32:109-112
Keywords
mousenuclear transferzona pellucida piercinglaser
Abstract
Somatic cells nuclear transfer (SCNT) is a useful tool in
studies of developmental biology and animal cloning. However,
SCNT experiments only are allowed to skilled technical
experts. In this experiment, laser-assisted zona pellucida
piercing tool (LASER) was applied in murine SCNT.
LASER minimized the use of piezo-driven micromanipulator
(PIEZO), reducing chances of problems caused
by PIEZO pulses. LASER reduced time that took to pierce
zona pellucida in removal of nucleus from oocyte and
somatic cell injection, which might have taken longer time
with PIEZO. Time and difficulties that took researcher of
equivalent skilled for their experiments were decreased
with LASER, and this might affect the improvement of
embryonic development. (LASER, 6.2% versus PIEZO,
2.9%; P< 0.05). Thus, these data support that the use of
LASER can be used for zona pellucida piercing in murine
SCNT program as an alternative to PIEZO.
ISSN
1226-7155
Language
English
URI
https://hdl.handle.net/10371/68599
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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