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In vitro expression of matrix metalloproteinase-1, tissue inhibitor of metalloproteinase-1 and transforming growth factor-beta 1 in human periodontal ligament fibroblasts
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Baek, Seung-Hak | - |
dc.contributor.author | Nahm, Dong-Seok | - |
dc.contributor.author | Kim, Hee-Jeong | - |
dc.contributor.author | Mah, James | - |
dc.date.accessioned | 2010-07-23 | - |
dc.date.available | 2010-07-23 | - |
dc.date.issued | 2004-04 | - |
dc.identifier.citation | European Journal of Orthodontics 26: 129-135 | en |
dc.identifier.issn | 0141-5387 | - |
dc.identifier.uri | https://hdl.handle.net/10371/68659 | - |
dc.description.abstract | Extracellular matrix remodelling is mediated via matrix metalloproteinases (MMPs) and their regulatory factors such as tissue inhibitors of metalloproteinases (TIMPs) and transforming growth factor-ss (TGF-ss). The regulation of MMPs is thought to be associated with cytoskeletal changes. In this study, cytoskeletal changes in human periodontal ligament fibroblasts (PDFs) were induced using cytochalasin B (CB) which reorganizes actin microfilaments reversibly, and colchicine which disrupts microtubules irreversibly. The levels of MMP-1, TIMP-1 and TGF-ss secreted by the CB- or colchicine-treated PDFs were measured using an enzyme-linked immunosorbent assay. Differences between experimental and control groups were tested using analysis of variance (ANOVA) and Scheffe's ad hoc test.
Although CB treatment did not significantly increase MMP-1 expression over the controls, colchicine treatment significantly increased the expression of MMP-1 (P < 0.01) in a time-dependent manner compared with the controls. Both CB and colchicine showed a time-dependent increase in TIMP-1 and TGF-ss1 expression and a dose-dependent increase in TIMP-1 and TGF-ss1 expression until threshold compared with the controls (P < 0.05, P < 0.01 and P < 0.001). In addition, CB treatment produced significantly increased TGF-ss1 expression over the controls (P < 0.05 and P < 0.001) from lower doses, with this effect occurring at earlier time points compared with colchicine treatment. | en |
dc.language.iso | en | en |
dc.publisher | Oxford University Press | en |
dc.title | In vitro expression of matrix metalloproteinase-1, tissue inhibitor of metalloproteinase-1 and transforming growth factor-beta 1 in human periodontal ligament fibroblasts | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 백승학 | - |
dc.contributor.AlternativeAuthor | 남동석 | - |
dc.contributor.AlternativeAuthor | 김희정 | - |
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