Allele Frequency and Genotype Distribution of STR SE33 locus in Koreans

Abstract

DNA was obtained from placental tissue and peripheral blood of 268 unrelated Koreans in order to analyze the applicability of PCR amplification of human beta-actin related pseudogene H-beta-Ac-psi-2 (AClBP-2, SE33) SlR locus for forensic medicine in the field of individual identification and paternity tests. After amplification using two primers specific to the SE33 locus, the products were analyzed by polyacrylmide gel electrophoresis, so called Amp-FLP(Amplification Fragment Length Polymorphism) procedure followed by silver staining. To improw the differentiation of the allele an automatic sequencer, Licor 4000-L was used and the result was compared with that of routine acrylamide gel electrophoresis. 36 alleles were deteced, and the distribution was somewhat homogenous, and none of the alleles were above 0.1 in frequency. Allele 12 and allele 14 were most frequent with frequencies of 0.09701 and 0.08022, and alleles of two base repeat such as allele 17, allele 19 were also encountered. 144 kinds of genotypes were found, among which 22 were homozygotes, and the heterozygosity was 91.85%. In 164 gametes of 64 families whose parent-child relationship were confirmed through other studies and information, Mendelian inheritance was well presented although two mutations were observed,