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NFI-C is essential for odontogenic cell proliferation and odontoblast differentiation during tooth root development

Cited 73 time in Web of Science Cited 73 time in Scopus
Authors
Park, Joo-Cheol; Lee, Dong-Seol; Park, Jong-Tae; Kim, Hyun-Man; Ko, Jea Seung; Son, Ho-Hyun; Gronostajski, Richard M; Cho, Moon-Il; Choung, Pill-Hoon
Issue Date
2009-04
Publisher
American Society for Biochemistry and Molecular Biology
Citation
Journal of Biological Chemistry 284, 17293-17303
Abstract
Our previous studies have demonstrated that nuclear factor I-C (NFI-C) null mice developed short molar roots that contain aberrant odontoblasts and abnormal dentin formation. Based on these findings, we performed studies to elucidate the function of NFI-C in odontoblasts. Initial studies demonstrated that aberrant odontoblasts become dissociated and trapped in an osteodentin-like mineralized tissue. Abnormal odontoblasts exhibit strong bone sialoprotein expression but a decreased level of dentin sialophosphoprotein expression when compared with wild type odontoblasts. Loss of Nfic results in an increase in p-Smad2/3 expression in aberrant odontoblasts and pulp cells in the subodontoblastic layer in vivo and primary pulp cells from Nfic-deficient mice in vitro. Cell proliferation analysis of both cervical loop and ectomesenchymal cells of the Nfic-deficient mice revealed significantly decreased proliferative activity compared with wild type mice. In addition, Nfic-deficient primary pulp cells showed increased expression of p21 and p16 but decreased expression of cyclin D1 and cyclin B1, strongly suggesting cell growth arrest caused by a lack of Nfic activity. Analysis of the pulp and abnormal dentin in Nfic-deficient mice revealed an increase in apoptotic activity. Further, Nfic-deficient primary pulp cells exhibited an increase in caspase-8 and -3 activation, whereas the cleaved form of Bid was hardly detected. These results indicate that the loss of Nfic leads to the suppression of odontogenic cell proliferation and differentiation and induces apoptosis of aberrant odontoblasts during root formation, thereby contributing to the formation of short roots.
ISSN
0021-9258
Language
English
URI
https://hdl.handle.net/10371/68815
DOI
https://doi.org/10.1074/jbc.M109.009084
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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