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High Extracellular Calcium Increased Expression of Ank, PC-1 andOsteopontin in Mouse Calvarial Cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Gwan-Shik | - |
dc.contributor.author | Song, Mina | - |
dc.contributor.author | Ryoo, Hyun-Mo | - |
dc.contributor.author | Woo, Kyung-Mi | - |
dc.contributor.author | Baek, Jeong-Hwa | - |
dc.date.accessioned | 2010-08-02 | - |
dc.date.available | 2010-08-02 | - |
dc.date.issued | 2008-03 | - |
dc.identifier.citation | International Journal of Oral Biology 33:33-43 | en |
dc.identifier.issn | 1226-7155 | - |
dc.identifier.uri | https://hdl.handle.net/10371/68816 | - |
dc.description.abstract | In the process of bone remodeling, mineral phase of bone
is dissolved by osteoclasts, resulting in elevation of calcium concentration in micro-environment. This study was performed to explore the effect of high extracellular calcium (Ca 2+ e) on mineralized nodule formation and on the expression of progressive ankylosis (Ank), plasma cell membrane glycoprotein-1 (PC-1) and osteopontin by primary cultured mouse calvarial cells. Osteoblastic differentiation and mineralized nodule formation was induced by culture of mouse calvarial cells in osteoblast differentiation medium containing ascorbic acid and β-glycerophosphate. Although Ank, PC-1 and osteopontin are well known inhibitors of mineralization, expression of these genes were induced at the later stage of osteoblast differentiation during when expression of osteocalcin, a late marker gene of osteoblast differentiation, was induced and mineralization was actively progressing. High Ca 2+ e (10 mM) treatment highly enhanced mRNA expression of Ank, PC-1 and osteopontin in the late stage of osteoblast differentiation but not in the early stage. Inhibition of p44/42 MAPK activation but not that of protein kinase C suppressed high Ca 2+ e-induced expression of Ank, PC-1 and osteopontin. When high Ca 2+ e (5 mM or 10 mM) was present in culture medium during when mineral deposition was actively progressing, matrix calcifiation was significantly increased by high Ca 2+ e. This stimulatory effect was abolished by pyrophosphate (5 mM) or levamisole (0.1-0.5 mM), an alkaline phosphatase inhibitor. In addition, probenecid (2mM), an inhibitor of Ank, suppressed matrix calcification in both control and high Ca 2+ e-treated group, suggesting the possible role of Ank in matrix calcification by osteoblasts. Taken together, these results showed that high Ca 2+ e stimulates expression of Ank, PC-1 and osteopontin as well as matrix calcification in late differentiation stage of osteoblasts and that p44/42 MAPK activation is involved in high Ca 2+ e- induced expression of Ank, PC-1 and osteopontin. | en |
dc.language.iso | ko | en |
dc.publisher | Korean Academy of Oral Biology | en |
dc.subject | high extracellular calcium | en |
dc.subject | osteoblast | en |
dc.subject | matrix calcification | en |
dc.subject | Ank | en |
dc.subject | PC-1 | en |
dc.subject | Osteopontin | en |
dc.title | High Extracellular Calcium Increased Expression of Ank, PC-1 andOsteopontin in Mouse Calvarial Cells | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 김관식 | - |
dc.contributor.AlternativeAuthor | 송미나 | - |
dc.contributor.AlternativeAuthor | 노현무 | - |
dc.contributor.AlternativeAuthor | 우경미 | - |
dc.contributor.AlternativeAuthor | 백정화 | - |
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