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Investigation of multipotent postnatal stem cells from human periodontal ligament

Cited 2255 time in Web of Science Cited 2419 time in Scopus
Issue Date
2004-07
Publisher
Elsevier
Citation
Lancet 2004; 364: 149-155
Abstract
Background Periodontal diseases that lead to the destruction of periodontal tissues--including periodontal ligament (PDL), cementum, and bone--are a major cause of tooth loss in adults and are a substantial public-health burden worldwide. PDL is a specialized connective tissue that connects cementum and alveolar bone to maintain and support teeth in situ and preserve tissue homeostasis. We investigated the notion that human PDL contains stem cells that could be used to regenerate periodontal tissue. Methods PDL tissue was obtained from 25 surgically extracted human third molars and used to isolate PDL stem cells (PDLSCs) by single-colony selection and magnetic activated cell sorting. Immunohistochemical staining, RT-PCR, and northern and western blot analyses were used to identify putative stem-cell markers. Human PDLSCs were transplanted into immunocompromised mice (n=12) and rats (n=6) to assess capacity for tissue regeneration and periodontal repair. Findings PDLSCs expressed the mesenchymal stem-cell markers STRO-1 and CD146/MUC18. Under denied culture conditions, PDLSCs differentiated into cementoblast-like cells, adipocytes, and collagen-forming cells. When transplanted into immunocompromised rodents, PDLSCs showed the capacity to generate a cementum/PDL-like structure and contribute to periodontal tissue repair. Interpretation Our findings suggest that PDL contains stem cells that have the potential to generate cementum/PDL-like tissue in vivo. Transplantation of these cells, which can be obtained from an easily accessible tissue resource and expanded ex vivo, might hold promise as a therapeutic approach for reconstruction of tissues destroyed by periodontal diseases
ISSN
0099-5355
Language
English
URI
https://hdl.handle.net/10371/68886
DOI
https://doi.org/10.1016/S0140-6736(04)16627-0
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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