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Kinetin Enhances In Vitro Development of Parthenogenetic and Nuclear Transfer Porcine Embryos

Cited 7 time in Web of Science Cited 7 time in Scopus
Authors
Min, B.-M; Won, C; Park, S.K; Cho, S.-G; Roh, S
Issue Date
2008-04
Publisher
Wiley-Blackwell
Citation
Molecular Reproduction and Development 75:1701-1709
Keywords
kinetinporcine embryoin vitro cultureparthenogenesisnuclear transfer
Abstract
Culture conditions affect the development of mammalian embryos in vitro. Kinetin belongs to the family of N6-substituted adenine derivates and promotes cell division, synthesis of DNA repair enzymes, superoxide dismutase activity, and ribosomal RNA transcription. We investigated the effects of kinetin on in vitro development of parthenogenetic and nuclear transfer (NT) porcine embryos. These embryos were cultured with or without kinetin in either BSA- or polyvinyl alcohol-containing medium for 7 days. mRNA expression of three developmentally important genes, HSP70, Glut-1, and poly[A] polymerase in NT embryos was analyzed. Regardless of kinetin supplementation, the proportion of blastocysts and blastocyst cells were not significantly different in parthenogenetic embryos. However, kinetin supplementation increased expansion and hatching rates in all groups. In somatic cell NT embryos, kinetin increased the proportion of embryos developed to blastocysts from 7.5% to 15.4% in medium supplemented with PVA. However, gene expression levels of HSP70, poly[A] polymerase and Glut-1 mRNA were not significantly different in NT blastocysts. The present study indicates that kinetin not only improves blastocyst expansion and cell number of parthenogenetic porcine embryos but also enhances NT porcine embryo development in a completely defined culture condition in vitro. Mol. Reprod. Dev. 75: 1701-1709, 2008. © 2008 Wiley-Liss, Inc.
ISSN
1040-452X
Language
English
URI
https://hdl.handle.net/10371/68887
DOI
https://doi.org/10.1002/mrd.20920
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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