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Characterization of Intermediate Conductance K+ Channels in Submandibular Gland Acinar Cells

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Issue Date
2002-12
Publisher
Korean Physiological Society = 대한생리학회
Citation
Korean Journal of Physiology & Pharmacology 6(6): 305-309
Abstract
There are some evidences that K+ efflux evoked by muscarinic stimulation is not mainly mediated
by large conductance K+ (BK) channels in salivary gland. In this experiment, we therefore characterised
non BK channels in rat submandibular gland acinar cells and examined the possibility of agonist effect
on this channel using a patch clamp technique. Two types of K+ channels were observed in these cells.
BK channels were observed in 3 cells from total 6 cells and its average conductance was 152±7 pS
(n=3). The conductance of the another types of K+ channel was estimated as 71±7 pS (n=6). On the
basis of the conductance of this channel, we defined this channel as intermediate conductance K+ (IK)
channels, which were observed from all 6 cells we studied. When we increased Ca2+ concentration of
the bath solution in inside-out mode, the IK channel activity was greatly increased, suggesting this
channel is Ca2+ sensitive. We next examined the effect of carbachol (CCh) and isoproterenol on the
activity of the IK channels. 10-5 M isoproterenol significantly increased the open probability (Po) from
0.08±0.02 to 0.21±0.03 (n=4, P<0.05). Application of 10-5 M CCh also increased Po from 0.048±0.03
to 0.55±0.33 (n=5, P<0.05) at the maximum channel activity. The degree of BK channel activation
induced by the same concentration of CCh was lower than that of IK channels; Po value was 0.011±0.003
and 0.027±0.005 in control and during CCh stimulation (n=3), respectively. The result suggests that
IK channels exist in salivary acinar cells and its channel activity is regulated by muscaricinic and β-
adrenergic agonist. We conclude that IK channels also play a putative role in secretion as well as the
BK channels in rat submandibular gland acinar cells.
ISSN
1226-4512
Language
English
URI
https://hdl.handle.net/10371/68911
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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