Anabaena variabilis의 수소 생산에 관한 광효율 측정

Abstract

Molecular hydrogen is a nonpolluting fuel that may play a role in the future energy economy. Cyanobacteria are efficient systems for the production of H2 from water using solar energy. The efficiency was defined by the ratio of produced H2 energy to absorbed light energy. To measure the absorbed light energy, IR and opalescent plate method was used. Cyanobacteria absorbed the light in a visible range but not in an infrared range. Therefore, IR can be used for measuring the net amount of light energy absorbed by cells. For measuring the conversion efficiency from light energy to H2 energy, a rectangular biochamber was used. Quantum meter and Radiometer was used to measuring the light intensity transfer the chamber. Cyanobacteria Anabaena variabilis was cultured at BG 11 with 3.6mM NaNO3 medium and the light intensity was 40~50 μmol/m2/s at growth phase and 120~140 μmol/m2/s at hydrogen production phase. The maximum hydrogen production was 50 mL for 42 hours and growth density of 1.15 g/L was observed. Photoproduction of hydrogen at rates of 4.14 mL H2/g dry cell weight/h was observed and conversion efficiency from light to hydrogen energy was 1.6% on average and 3.9% at maximum.