S-Space College of Engineering/Engineering Practice School (공과대학/대학원) Dept. of Chemical and Biological Engineering (화학생물공학부) Theses (Master's Degree_화학생물공학부)
Expression of olfactory receptor protein in e.coli and its application to olfactory biosensor
- Issue Date
- 서울대학교 대학원
- 후각수용체 단백질; Olfactory receptor; ODR-10; Odr-10; 전자코; Qcm; 후각센서; Electronic nose; QCM; Piezoelectric
- Thesis (master`s)--서울대학교 대학원 :응용화학부,2003.
- An olfactory receptor protein is a 7-transmembrane protein that is
expressed at the surface of olfactory sensory neuron. It is known to bind
to odorant molecules and constitute the first step in sense of smell. Since
the discovery of olfactory receptor gene in 1991, it is now known that
there are as many as 1000 genes for olfactory receptors in the mammalian
genome, probably being the largest gene family of GPCR(G-protein
coupled receptor). Since thousands of odorant molecules are detected by
about 1000 olfactory receptors, there have been some research interests
on how such a large number of odors can be detected by a limited number
of olfactory receptors. Also, the olfactory system and olfactory receptors
are excellent models for structure-function studies, and there have been
studies on identification of odorant ligands for several olfactory receptors.
But these studies have not shown much progress, due to the difficulty of
expressing olfactory receptors in heterologous systems.
In our studies, olfactory receptor protein ODR-10 was expressed in
E. coli as fusion protein with GST and 6XHis Tag. GST(glutathione Stransferase)
and 6XHis genes were fused to 5 and 3 end of odr-10 gene,
respectively. Expression of the target protein was checked by SDS-PAGE
and western blot, and it was shown that the protein was expressed at the membrane fraction of the host cells, although the expression level was
- Files in This Item: There are no files associated with this item.