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Specific activation of the human HSP70 promoter by copper sulfate in mosaic transgenic zebrafish

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dc.contributor.authorSeok, Seung-Hyeok-
dc.contributor.authorPark, Jong-Hwan-
dc.contributor.authorBaek, Min-Won-
dc.contributor.authorLee, Hui-Young-
dc.contributor.authorKim, Dong-Jae-
dc.contributor.authorUhm, Hyung-Min-
dc.contributor.authorHong, Jung-Joo-
dc.contributor.authorNa, Yi-Rang-
dc.contributor.authorJin, Bo-Hwan-
dc.contributor.authorRyu, Doug Young-
dc.contributor.authorPark, Jae Hak-
dc.date.accessioned2009-08-11T03:29:34Z-
dc.date.available2009-08-11T03:29:34Z-
dc.date.issued2006-05-30-
dc.identifier.citationJ. Biotechnol. 126, 406–413en
dc.identifier.issn0168-1656-
dc.identifier.urihttps://hdl.handle.net/10371/6900-
dc.description.abstractHeat shock proteins (HSPs) play a central role in cell protection and repair upon stresses, such as that caused by heat and heavy metals. Copper sulfate inducibility of a pHhsp70 construct expressing the enhanced green fluorescent protein (EGFP) gene under the control of the exogenous human hsp70 promoter was tested in transfected CHSE 214 cells and transgenic zebrafish (Danio rerio). We developed a transient expression system, using mosaically transgenic zebrafish, which allows rapid analysis of transgenic expression. Transfected CHSE 214 cells which had been exposed to 250 nM and 2.5 μM copper sulfate for up to 24 h showed increased EGFP expression in a dose-dependent manner. The 1.5 μM copper sulfate caused stronger EGFP fluorescence than the 1.0 μM copper sulfate in transgenic zebrafish. Most of the expression was spotty and was detected in the gills, dorsal and ventral retina, myotubes of the trunk, and skin epithelium. Transgenic zebrafish exposed to copper sulfate exhibited gross dysmorphogenesis, edema and trunk abnormalities, such as spinal lordosis, in vertebral development 5 days after fertilization. This transgenic zebrafish system was sensitive enough to detect copper sulfate at doses below the median lethal concentration (the LC50 was calculated to be 1.2 μM (95% confidence interval of 0.6–1.9 μM)). These results indicate that zebrafish could be useful transgenic biosensor systems for the detection of xenobiotic toxicants in the environment.en
dc.description.sponsorshipThis work was supported by a grant from the Korea
Institute of Environmental Science andTechnology and
a grant from the Brain Korea 21 Project. Additional
support was also provided by Korea Research Foundation
grants (KRF-005-E00077).
en
dc.language.isoen-
dc.publisherElsevieren
dc.subjectCopper sulfateen
dc.subjectGreen fluorescent proteinen
dc.subjectHuman hsp70 promoteren
dc.subjectTransgenic zebrafishen
dc.titleSpecific activation of the human HSP70 promoter by copper sulfate in mosaic transgenic zebrafishen
dc.typeArticleen
dc.contributor.AlternativeAuthor석승혁-
dc.contributor.AlternativeAuthor박종환-
dc.contributor.AlternativeAuthor백민원-
dc.contributor.AlternativeAuthor이희영-
dc.contributor.AlternativeAuthor김동재-
dc.contributor.AlternativeAuthor엄형민-
dc.contributor.AlternativeAuthor홍정주-
dc.contributor.AlternativeAuthor나이랑-
dc.contributor.AlternativeAuthor진보환-
dc.contributor.AlternativeAuthor류덕영-
dc.contributor.AlternativeAuthor박재학-
dc.identifier.doi10.1016/j.jbiotec.2006.04.029-
Appears in Collections:
College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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