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Unique features of bovine lymphocytes exposed to a staphylococcal enterotoxin

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Authors
Park, Yong Ho; Lee, Sang Un; Ferens, Witold A.; Samuels, Sparrow; Davis, Sparrow; Fox, Lawrence K.; Ahn, Jong Sam; Seo, Keun Seok; Chang, Byoung Sun; Hwang, Sun Young; Bohach, Gregory A.
Issue Date
2006
Publisher
대한수의학회 = The Korean Society of Veterinary Science
Citation
J Vet Sci 2006, 7, 233-239
Keywords
bovineenterotoxinmastitisStaphylococcus aureussuperantigen
Abstract
We previously demonstrated that stimulation of bovine
peripheral blood mononuclear cells (PBMCs) with
staphylococcal enterotoxin C (SEC), led to an inversion of
the CD4+:CD8+ T cell ratio and generation of an atypical
CD8+ T cell subpopulation expressing CD26. In the present
study, we examined T cell apoptosis and proliferation
profiles of PBMC subpopulations in cultures stimulated
with SEC. Unlike when stimulated with concanavalin A,
nucleic acid synthesis in bovine PBMC cultures stimulated
with SEC was low during the first four days but increased
greatly on day 5. In contrast, nucleic acid synthesis in
human PBMC cultures stimulated with SEC increased
continuously. To investigate the mechanism of delayed
bovine T cell proliferation, various cell phenotypes were
monitored. The inversion of the bovine CD4+:CD8+ T cell
ratio in PBMC cultures stimulated by SEC was associated
with higher proliferation and lower apoptosis of CD8+ T
cells compared to CD4+ T cells. The mRNA levels for
interleukin (IL)-4 and IL-13 were sustained over 4 days
but IL-12 mRNA levels dropped to background on day 2.
These data suggest that SEC induces a prolonged Th-2-
biased microenvironment, and together with the inversion
of the bovine CD4+:CD8+ T cell ratios in bovine PBMC
cultures with SEC, may in part explain the inability of the
mammary immune system to establish an effective
response to Staphylococcus aureus infections.
ISSN
1229-845X:
Language
English
URI
http://www.vetsci.org/2006/abstract/233a.html

https://hdl.handle.net/10371/6920
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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