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Regenerated Silk Fibroin Nanofibers: Water Vapor-Induced Structural Changes and Their Effects on the Behavior of Normal Human Cells

Cited 128 time in Web of Science Cited 129 time in Scopus
Authors
Min, Byung-Moo; Jeong, Lim; Lee, Kuen Yong; Park, Won Ho
Issue Date
2006-03
Publisher
Wiley-Blackwell
Citation
Macromolecular Bioscience 2006, 6, 285-292
Keywords
b-sheet conformationcell behaviornanofibersilk fibroinstructural changes
Abstract
Summary: Nanofibrous non-woven matrices were prepared by electrospinning a regenerated silk fibroin (SF) solution, and the structural changes of SF nanofibers treated with water vapor were investigated using time-resolved IR and 13C CP/MAS NMR spectroscopy. Conformational transitions of SF from random coil to -sheet structures were induced by water vapor treatment and were strongly dependent on the treatment time and temperature. Water vapor treatment provided a useful means of stabilizing the SF nanofiber matrices, resulting in the formation of matrices with a decreased solubility in water and increased mechanical strength. The adhesion and spreading of both normal human keratinocytes and fibroblasts onto the SF nanofiber matrices were also investigated, and the water vapor-treated SF nanofiber matrices showed good cellular compatibility, in comparison with traditional methanol-treated ones. This approach to controlling the conformational changes of SF nanofibers by water vapor treatment may be useful in the design and tailoring of novel materials for biomedical applications, including wound dressings and scaffolds for tissue engineering.
ISSN
1616-5187
Language
English
URI
https://hdl.handle.net/10371/69604
DOI
https://doi.org/10.1002/mabi.200500246
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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