Browse

Terminal differentiaiton of normal human oral keratinocytes is associated with enhanced cellular TGF-β and phospholipase C-γ1 levels, and apoptotic cell death

Cited 49 time in Web of Science Cited 44 time in Scopus
Authors
Min, Byung-Moo; Woo, Kyung Mi; Lee, Gene; Park, No-Hee
Issue Date
1999-06
Publisher
Elsevier
Citation
Experimental Cell Research 249, 377-385
Keywords
TGF-βphospholipase C-γ1apoptosisterminal differentiationnormal human oral keratinocytes
Abstract
Subculture of primary normal human oral keratinocytes (NHOK) results in terminal differentiation, leading to cell death. To investigate whether the subculture-induced death of NHOK is due to apoptosis, we studied transferase-mediated dUTP nick end labeling (TUNEL)-positive cells, DNA fragmentation, and expression of several apoptosis-associated genes from NHOK with different passage numbers. We also determined the effect of transforming growth factor β1 (TGF-β1) on the induction of apoptosis in NHOK. We were able to subculture primary NHOK up to the fifth passage, at which point cells showed morphological features of differentiation. Appearance of DNA fragmentation concurrently occurred with an increase in the number of TUNEL-positive cells with higher passage numbers. The level of cellular p53 proteins was gradually decreased by the continued passage of cells, whereas the levels of intracellular and secreted TGF-β and phospholipase C-γ1 (PLC-γ1) were significantly elevated by serial subculture. Exogenous TGF-β1 also induced differentiation and apoptosis of proliferating NHOK. These data indicate that terminal differentiation of NHOK is associated with apoptosis, which is, in part, linked to elevated cellular levels of TGF-β and PLC-γ1.
ISSN
0014-4827
Language
English
URI
https://hdl.handle.net/10371/69630
DOI
https://doi.org/10.1006/excr.1999.4489
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse