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The bone regenerative effect of platelet derived growth factor-BB delivered with a chitosan/tricalcium phospahate matrix carrier

Cited 108 time in Web of Science Cited 121 time in Scopus
Authors
Ku, Young; Lee, Yong-Moo; Park, Yoon-Jeong; Lee, Seung-Jin; Han, Soo-Boo; Klokkevold, Perry R; Chung, Chong-Pyoung
Issue Date
2000
Publisher
American Academy of Periodontology
Citation
Journal of Periodontology 2000;71:418-424
Keywords
Bone regenerationanimal studiesbone developmentchitosangrowth factorsplatelet-derivedtricalcium phosphatespongesartificialtissue engineering
Abstract
Background: In order to achieve optimal effects, growth factors including platelet-derived growth factor (PDGF) should be delivered with a biodegradable carrier that will release therapeutic concentrations over a sufficient length of time. The purpose of this study was to evaluate the bone regenerative effect of PDGF-BB delivered with a chitosan/tricalcium phosphate (TCP) sponge carrier in a rat calvarial defect model.

Methods: The PDGF-BB–loaded chitosan/TCP sponge carrier was fabricated by freeze-drying a mixture of chitosan solution and TCP powder and soaking in a PDGF-BB solution. The release kinetics of PDGF-BB loaded onto the sponge were measured in vitro with 125I-labeled PDGF-BB. Chitosan/TCP sponges with and without PDGF-BB were implanted into 8 mm calvarial defects in rats. Rats were sacrificed at 2 and 4 weeks following implantation, and histologic and histomorphometrical examinations were performed.c

Results: In vitro evaluation demonstrated that an effective therapeutic concentration of PDGF-BB following a high initial burst release was maintained throughout the examination period. In the histologic examination, the chitosan/TCP sponge carrier promoted osseous healing of the rat calvarial defects as compared to controls. The addition of PDGF-BB to the carrier further enhanced bone regeneration. Evidence of the degraded sponge matrix was observed mingled within the newly formed bone without connective tissue encapsulation.

Conclusions: The results of this study support the use of chitosan/ TCP sponges as a delivery system for growth factors and demonstrate that PDGF-BB loaded onto chitosan/TCP sponge carriers has an osteogenic effect on bone regeneration in vivo. J Periodontol 2000;71:418-424.
ISSN
0022-3492
Language
English
URI
https://hdl.handle.net/10371/69635
DOI
https://doi.org/10.1902/jop.2000.71.3.418
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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