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The differentiation-inducing effect of conditioned media obtained from dental pulp cells

Cited 8 time in Web of Science Cited 9 time in Scopus
Issue Date
2009-05
Publisher
Elsevier
Citation
Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontics 2009;107:e54-e59
Abstract
Objective
Conditioned media (CM) from human dental pulp cells (HDPC) was investigated for its effects on the proliferation and differentiation of HDPC and MG63 cells.

Study design
CM prepared from the primary culture of HDPC was used for the culture of HDPC and MG63. Cell growth, alkaline phosphatase (ALPase) activity, mRNA expression of differentiation-related genes, and mineralization of the HDPC and MG63 cells in the media containing CM were assessed.

Results
HDPC CM increased the cell growth of HDPC but decreased MG63 cell growth. ALPase activity and the mineralization of both HDPC and MG63 were enhanced by HDPC CM. The CM also up-regulated the expressions of DSPP, DMP-1, and OCN mRNA in HDPC. Pretreatment of HDPC CM with a neutralizing antibody against TGF-β completely eliminated the effect of CM on ALPase activity in HDPC.

Conclusion
HDPC was able to secrete odontogenic differentiation-inducing factors, in which TGF-β seems to a key element of the CM effects.
ISSN
1079-2104
Language
English
URI
https://hdl.handle.net/10371/69649
DOI
https://doi.org/10.1016/j.tripleo.2009.01.047
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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