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The triacylated ATP binding cluster transporter substrate-binding lipoprotein of Staphylococcus aureus functions as a native ligand for the toll-like receptor 2.

Cited 107 time in Web of Science Cited 106 time in Scopus
Authors
Kurokawa, Kenji; Lee, Hanna; Roh, Kyung-Baeg; Asanuma, Miwako; Kim, Young Sook; Nakayama, Hiroshi; Shiratsuchi, Akiko; Choi, Youngnim; Takeuchi, Osamu; Kang, Hee Jung; Dohmae, Naoshi; Nakanishi, Yoshinobu; Akira, Shizuo; Sekimizu, Kazuhisa; Lee, Bok Luel
Issue Date
2009-03
Publisher
American Society for Biochemistry and Molecular Biology
Citation
JOURNAL OF BIOLOGICAL CHEMISTRY. 284, 8406-8411
Abstract
Some synthetic lipopeptides, in addition to native lipoproteins derived from both Gram-negative bacteria and mycoplasmas, are known to activate TLR2 (Toll-like receptor 2). However, the native lipoproteins inherent to Gram-positive bacteria, which function as TLR2 ligands, have not been characterized. Here, we have purified a native lipoprotein to homogeneity from Staphylococcus aureus to study as a native TLR2 ligand. The purified 33-kDa lipoprotein was capable of stimulating TLR2 and was identified as a triacylated SitC lipoprotein, which belongs to a family of ATP binding cluster (ABC) transporter substrate-binding proteins. Analyses of the SitC-mediated production of cytokine using mouse peritoneal macrophages revealed that the SitC protein (3 nm) induced the production of tumor necrosis factor-α and interleukin-6. Moreover, analysis of knock-out mice showed that SitC required TLR2 and MyD88, but not TLR1 or TLR6, for the induction of cytokines. In addition to the S. aureus SitC lipoprotein, we purified two other native ABC transporter substrate-binding lipoproteins from Bacillus subtilis and Micrococcus luteus, which were both shown to stimulate TLR2. These results demonstrate that S. aureus SitC lipoprotein is triacylated and that the ABC transporter substrate-binding lipoproteins of Gram-positive bacteria function as native ligands for TLR2.
ISSN
0021-9258
Language
English
URI
https://hdl.handle.net/10371/69664
DOI
https://doi.org/10.1074/jbc.M809618200
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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