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Tumor necrosis factor-alpha induces differentiation of human peripheral blood mononuclear cells into osteoclasts through the induction of p21(WAF1/Cip1).
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kwak, Han Bok | - |
dc.contributor.author | Jin, Hye-Mi | - |
dc.contributor.author | Ha, Hyunil | - |
dc.contributor.author | Kang, Mi-Jin | - |
dc.contributor.author | Lee, Seung Bok | - |
dc.contributor.author | Kim, Hong-Hee | - |
dc.contributor.author | Lee, Zang Hee | - |
dc.date.accessioned | 2010-09-03T06:07:33Z | - |
dc.date.available | 2010-09-03T06:07:33Z | - |
dc.date.issued | 2005-05 | - |
dc.identifier.citation | Biochemical and Biophysical Research Communications 330 (2005) 1080-1086 | en |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://hdl.handle.net/10371/69665 | - |
dc.description.abstract | Tumor necrosis factor-α (TNF-α) is a multifunctional cytokine that mediates inflammation and induces bone loss caused by excessive bone resorption by osteoclasts. The interaction of TNF-α with its receptor activates several signal transduction pathways, including those of mitogen-activated protein (MAP) kinases (p38, JNK, and ERK) and NF-κB. Signaling from these molecules has been shown to play an important role in osteoclastogenesis. In the present study, we investigated the mechanism of TNF-α-induced osteoclast differentiation in human peripheral blood mononuclear cells (PBMCs). We found that TNF-α alone greatly induced differentiation of PBMCs into osteoclasts. The osteoclast differentiation induced by TNF-α was independent of RANKL binding to its receptor RANK on PBMCs. Furthermore, TNF-α potently activated p38 MAPK, JNK, and NF-κB. Western blotting analysis revealed that p21WAF1/Cip1, a cyclin-dependent kinase (CDK) inhibitor, is significantly induced upon TNF-α stimulation. The induction of p21WAF1/Cip1 during differentiation is responsible for arrest at G0/G1 phase and associated with the JNK pathway. These results suggest that TNF-α regulates osteoclast differentiation through p21WAF1/Cip1 expression and further shows that these events require JNK activity. | en |
dc.language.iso | en | en |
dc.publisher | Elsevier | en |
dc.subject | TNF-α | en |
dc.subject | PBMC | en |
dc.subject | Osteoclast | en |
dc.subject | p21WAF1/Cip1 | en |
dc.title | Tumor necrosis factor-alpha induces differentiation of human peripheral blood mononuclear cells into osteoclasts through the induction of p21(WAF1/Cip1). | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 곽한복 | - |
dc.contributor.AlternativeAuthor | 진혜미 | - |
dc.contributor.AlternativeAuthor | 하현일 | - |
dc.contributor.AlternativeAuthor | 강미진 | - |
dc.contributor.AlternativeAuthor | 이승복 | - |
dc.contributor.AlternativeAuthor | 김홍희 | - |
dc.contributor.AlternativeAuthor | 이장희 | - |
dc.identifier.doi | 10.1016/j.bbrc.2005.03.085 | - |
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