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Trichostatin A-mediated upregulation of p21(WAF1) contributes to osteoclast apoptosis.

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Authors
Yi, TacGhee; Baek, Jeong-Hwa; Kim, Hye-Jin; Choi, Mi-Hye; Seo, Sang-Beom; Ryoo, Hyun-Mo; Kim, Gwan-Shik; Woo, Kyung Mi
Issue Date
2007-04
Publisher
Korean Society of Medical Biochemistry
Citation
EXPERIMENTAL AND MOLECULAR MEDICINE 2007;39:213-221
Abstract
Histone deacetylase inhibitors (HDIs), a new class of anti-cancer agents, have been reported to suppress formation of osteoclast precursors and their fusion into multinucleated cells. However, little is known about the effect of HDIs on mature osteoclasts, which may have significance for their therapeutic use. Here, we demonstrate a novel action of HDIs on osteoclast apoptosis. Primary multinucleated mature osteoclasts were prepared from mouse bone marrow cells. Treatment of osteoclasts with the HDI trichostatin A (TSA) caused apoptosis, as confirmed by annexin V staining and caspase activation. TSA caused the upregulation of p21WAF1 in osteoclasts. To understand the role of p21WAF1 upregulation in TSA-treated osteoclasts, shRNA against p21WAF1-containing lentivirus was introduced into osteoclasts. The suppression of p21WAF1 decreased TSA-directed osteoclast apoptosis. Collectively, our results provide evidence that TSA causes osteoclast apoptosis, which involves, in part, TSA-induced upregulation of p21WAF1, and strongly supports HDIs as potential therapeutic agents for excessive bone resorption.
ISSN
1226-3613
Language
English
URI
https://hdl.handle.net/10371/69669
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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