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Differential gene expression and lipid metabolism in fatty liver induced by acute ethanol treatment in mice

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dc.contributor.authorYin, Hu-Quan-
dc.contributor.authorKim, Mingoo-
dc.contributor.authorKim, Ju-Han-
dc.contributor.authorKong, Gu-
dc.contributor.authorKang, Kyung-Sun-
dc.contributor.authorKim, Hyung-Lae-
dc.contributor.authorYoon, Byung-Il-
dc.contributor.authorLee, Mi-Ock-
dc.contributor.authorLee, Byung-Hoon-
dc.date.accessioned2009-08-11T22:49:46Z-
dc.date.available2009-08-11T22:49:46Z-
dc.date.issued2007-07-04-
dc.identifier.citationToxicol. Appl. Pharmacol. 223 225-233en
dc.identifier.issn0041-008X-
dc.identifier.urihttps://hdl.handle.net/10371/6969-
dc.description.abstractEthanol induces cumulative liver damage including steatosis, steatohepatitis and cirrhosis. The aim of this study is to investigate the global intrahepatic gene expression profile in the mouse liver treated with ethanol. A single oral dose of 0.5 or 5 g/kg ethanol was administered to male ICR mice, and liver samples were obtained after 6, 24 and 72 h. Histopathological evaluation showed typical fatty livers in the high-dose group at 24 h. Microarray analysis identified 28 genes as being ethanol responsive (two-way ANOVA; p < 0.05), after adjustment by the Benjamini–Hochberg multiple testing correction; these genes displayed ≥ 2-fold induction or repression. The expression of genes that are known to be involved in fatty acid synthesis was examined. The transcript for lipogenic transcription factor, sterol regulatory element (SRE)-binding factor 1 (Srebf1), was upregulated by acute ethanol exposure. Of the genes known to contain SRE or SRE-like sequences and to be regulated by SRE-binding protein 1 (SREBP1), those encoding malic enzyme (Mod1), ATP-citrate lyase (Acly), fatty acid synthase (Fasn) and stearyl-CoA desaturase (Scd1) were induced by ethanol. Quantitative real-time PCR confirmed the changes in the expression levels of the selected genes. The change in the Srebf1 mRNA level correlates well with that of the SREBP1 protein expression as well as its binding to the promoters of the target genes. The present study identifies differentially expressed genes that can be applied to the biomarkers for alcohol-binge-induced fatty liver. These results support the hypothesis by which ethanol-induced steatosis in mice is mediated by the fatty acid synthetic pathway regulated by SREBP1.en
dc.description.sponsorshipThis work was supported by a Korea Food and Drug Administration grant (KFDA-05122-TGP-584).en
dc.language.isoenen
dc.publisherElsevieren
dc.subjectEthanolen
dc.subjectFatty liveren
dc.subjectToxicogenomicsen
dc.subjectFatty acid synthesisen
dc.subjectSterol regulatory element-binding protein (SREBP)en
dc.subjectMicroarrayen
dc.titleDifferential gene expression and lipid metabolism in fatty liver induced by acute ethanol treatment in miceen
dc.typeArticleen
dc.contributor.AlternativeAuthor김민구-
dc.contributor.AlternativeAuthor김주한-
dc.contributor.AlternativeAuthor공구-
dc.contributor.AlternativeAuthor강경선-
dc.contributor.AlternativeAuthor김형래-
dc.contributor.AlternativeAuthor윤병일-
dc.contributor.AlternativeAuthor이미옥-
dc.contributor.AlternativeAuthor이병훈-
dc.identifier.doi10.1016/j.taap.2007.06.018-
Appears in Collections:
College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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