Browse

The suppressive effect of myeloid Elf-1-like factor (MEF) in osteogenic differentiation

DC Field Value Language
dc.contributor.authorRyoo, Hyun-Mo-
dc.contributor.authorKim, Youn-Jeong-
dc.contributor.authorKim, Byung-Gyu-
dc.contributor.authorLee, Seung-Jin-
dc.contributor.authorLee, Ho-Kyung-
dc.contributor.authorLee, Sang-Han-
dc.contributor.authorCho, Je-Yoel-
dc.date.accessioned2010-09-06-
dc.date.available2010-09-06-
dc.date.issued2006-12-
dc.identifier.citationJOURNAL OF CELLULAR PHYSIOLOGY 211:253-260en
dc.identifier.issn0021-9541-
dc.identifier.urihttps://hdl.handle.net/10371/69712-
dc.description.abstractMyeloid Elf-1 like factor (MEF) is a member of the Ets transcription factor family. Ets family proteins control the expression of genes that are critical for biological processes such as proliferation, differentiation, and cell death. Some of Ets factors are also known to regulate bone development. In this study, we investigated the role of MEF in osteoblast differentiation. MEF expression was highest early in the differentiation of MC3T3-E1 osteoblasts and was reduced by treatment with BMP-2. The expression of MEF suppressed the alkaline phosphatase activity and expression induced by BMP-2 stimulation and mediated by Runx2. The expression of MEF also reduces osteocalcin mRNA levels, and mineralization in MC3T3-E1 cells. We found that the MEF-mediated suppression of osteogenic differentiation was critically related to Runx2 regulation. The MEF and Runx2 proteins physically interact to form a complex, and this interaction interferes with Runx2 binding to the cis-acting element OSE2 derived from the osteocalcin promoter. Co-transfection of MEF inhibited the 6xOSE2-luciferase reporter activity induced by Runx2. In addition, MEF stimulated the transcription of a negative mediator Msx2, and a transcriptional repressor, Mab21L1, and suppressed the transcription of a positive mediator, Dlx5 in osteoblast differentiation. MEF overexpression stimulated C2C12 cell proliferation. Together, our findings suggest that MEF promotes cell proliferation and functions as a negative regulator of osteogenic differentiation by directly interacting with Runx2 and suppressing its transcriptional activity. J. Cell. Physiol. 211: 253-260, 2007. © 2006 Wiley-Liss, Inc.en
dc.language.isoenen
dc.publisherWiley-Blackwellen
dc.titleThe suppressive effect of myeloid Elf-1-like factor (MEF) in osteogenic differentiationen
dc.typeArticleen
dc.contributor.AlternativeAuthor류현모-
dc.contributor.AlternativeAuthor김연정-
dc.contributor.AlternativeAuthor김병규-
dc.contributor.AlternativeAuthor이승진-
dc.contributor.AlternativeAuthor이호경-
dc.contributor.AlternativeAuthor이상한-
dc.contributor.AlternativeAuthor조재열-
dc.identifier.doi10.1002/jcp.20933-
Appears in Collections:
College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
Files in This Item:
There are no files associated with this item.
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse