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Enhanced bone augmentation by controlled release of recombinant human bone morphogenetic protein-2 from bioabsorbable membrane

Cited 39 time in Web of Science Cited 39 time in Scopus
Authors
Lee, Yong-Moo; Nam, Sung-Heon; Seol, Yang-Jo; Kim, Tae-Il; Lee, Seung-Jin; Ku, Young; Rhyu, In-Chul; Chung, Chong-Pyoung; Han, Soo-Boo; Choi, Sang-Mook
Issue Date
2003-06
Publisher
American Academy of Periodontology
Citation
JOURNAL OF PERIODONTOLOGY 2003;74:865-872
Keywords
Animal studiesbonecraniofacialL-polylactidemembranesbioabsorbableosteogenesisproteinsbone morphogenetictricalcium phosphate
Abstract
Background: The present study was undertaken to determine the effect of recombinant human bone morphogenetic protein- (rhBMP-2)-loaded biodegradable membranes on bone augmentation in a rabbit calvarial model.

Methods: Five µg of rhBMP-2 was loaded into a stiff hemispherical dome membrane made of poly(L-lactide) and tricalcium phosphate (PLLA/TCP). The release kinetics of rhBMP-2 from the membrane were determined in vitro using a human BMP-2 immunoassay. Twelve rhBMP-2-loaded dome membranes (test group) and 12 control dome membranes (control group) were placed on the partial-thickness calvarial defects of 24 rabbits. The animals were sacrificed at 4 and 8 weeks, and undecalcified ground sections were prepared. Newly formed bone area and height were measured histomorphometrically and calculated by percentage ratio to the total submembranous space area and height below the dome.

Results: In vitro release results demonstrated that rhBMP-2 was released consistently over a 4-week period following a high initial burst release on the first day. At both 4 and 8 weeks, histomorphometric analysis revealed that the test group showed signi ficantly higher newly formed bone heights and areas than the control group (P <0.01). In the control group, new bone height was 36.3% of the dome height and the new bone area reached 8.2% of the submembranous space area at 8 weeks, while the test group reached 87.3% and 35.4%, respectively.

Conclusion: These results suggest that the use of rhBMP- 2-loaded PLLA/TCP membranes can result in additional bone augmentation, which is due to the osteoinductive properties of rhBMP-2 released from the membrane during healing. J Periodontol 2003;74:865-872.
ISSN
0022-3492
Language
English
URI
https://hdl.handle.net/10371/69734
DOI
https://doi.org/10.1902/jop.2003.74.6.865
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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