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Birth of viable female dogs produced by somatic cell nuclear transfer
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jang, Goo | - |
dc.contributor.author | Kim, M.K. | - |
dc.contributor.author | Oh, H.J. | - |
dc.contributor.author | Hossein, M.S. | - |
dc.contributor.author | Fibrianto, Y.H. | - |
dc.contributor.author | Hong, S.G. | - |
dc.contributor.author | Park, J.E. | - |
dc.contributor.author | Kim, J.J. | - |
dc.contributor.author | Kim, H.J. | - |
dc.contributor.author | Kang, S.K. | - |
dc.contributor.author | Kim, Dae-Yong | - |
dc.contributor.author | Lee, Byeong Chun | - |
dc.date.accessioned | 2009-08-11T22:56:55Z | - |
dc.date.available | 2009-08-11T22:56:55Z | - |
dc.date.issued | 2006-12-13 | - |
dc.identifier.citation | Theriogenology 2007;67:941-947 | en |
dc.identifier.issn | 0093-691X | - |
dc.identifier.uri | https://hdl.handle.net/10371/6973 | - |
dc.description.abstract | Since the only viable cloned offspring born in dogs was a male, the purpose of the present study was to produce female puppies by somatic cell nuclear transfer (SCNT). Adult ear fibroblasts from a 2-month-old female Afghan hound were isolated and used as donor cells. In vivo-matured canine oocytes surgically collected (approximately 72 h after ovulation) from the oviducts of 23 donors were used for SCNT. After removal of the cumulus cells, oocytes were enucleated, microinjected, fused with a donor cell, and activated. A total of 167 reconstructed SCNT embryos were surgically transferred (Day 0) into the oviducts of 12 recipient bitches (average 13.9 embryos/recipient, range 6–22) with spontaneous, synchronous estrous cycles. Three pregnancies were detected by ultrasonography on Day 23, maintained to term, and three healthy female puppies (520, 460, and 520 g), were delivered by Caesarean section on Day 60. These puppies were phenotypically and genotypically identical to the cell donor. In conclusion, we have provided the first demonstration that female dogs can be produced by nuclear transfer of ear fibroblasts into enucleated canine oocytes. | en |
dc.description.sponsorship | This study was financially supported by KOSEF (grant #M10625030005-06N250300510). We thank K.S. Kim, owner of the female Afghan hound for donated donor cells and Y.H. Kim (NICEM) for assistance regarding microsatellite analysis. The authors are grateful for a graduate fellowship provided by the Korean MOE, through the BK21 program for Veterinary Science, SNU. | en |
dc.language.iso | en | - |
dc.publisher | Elsevier | en |
dc.subject | Female cloned dogs | en |
dc.subject | SCNT | en |
dc.subject | In vivo oocyte | en |
dc.subject | Embryo transfer | en |
dc.subject | Pregnancy | en |
dc.title | Birth of viable female dogs produced by somatic cell nuclear transfer | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 김대용 | - |
dc.identifier.doi | 10.1016/j.theriogenology.2006.11.006 | - |
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