S-Space College of Dentistry/School of Dentistry (치과대학/치의학대학원) Dept. of Dentistry (치의학과) Journal Papers (저널논문_치의학과)
Activation of phospholipase D by surface roughness of titanium in MG63 osteoblast-like cell
- Kim, Myung-Joo; Choi, Myung-Un; Kim, Chang-Whe
- Issue Date
- Biomaterials 2006;27:5502–5511
- Ti surface roughness; Osteoblast differentiation; Alkaline phosphatase activity; Osteocalcin production; Phospholipase D
- Although it is recognized that the surface roughness of titanium (Ti) promotes the osteogenic differentiation, the related mechanisms
and factors remain elusive. The purpose of this study was to explore the potential correlation among phospholipase D (PLD) activity, Ti
surface roughness and subsequent osteoblast differentiation. The machined Ti disks were sandblasted with aluminum oxide particles to
produce surfaces of varying roughness (n ¼ 160). Normal or transfected MG63cells with PLD genes were cultured on roughened Ti
specimens and assayed for PLD, alkaline phosphatase (ALP) and osteocalcin. The statistical significance was evaluated by analysis of
variance. The activity, mRNA and protein levels of PLD significantly increased in MG63 cells with a roughness-dependent pattern
(Po0:05). The ALP activity and osteocalcin production, promoted by Ti surface roughness, were enhanced by the PLD activator and
inhibited by the PLD blocker. It was also found that the PLD1 isoform responds to Ti surface roughness and regulates selectively the
ALP activity. These observations strongly suggest that PLD1 mediates the cellular signaling of and modulates osteoblast differentiation
induced by Ti surface roughness in MG63 osteoblast-like cell.
r 2006 Elsevier Ltd. All rights reserved.
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